{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T05:12:37Z","timestamp":1776834757942,"version":"3.51.2"},"reference-count":75,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,11,9]],"date-time":"2020-11-09T00:00:00Z","timestamp":1604880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cytomegalovirus (CMV)\u2010based vaccines show promising effects against chronic infections in nonhuman primates. Therefore, we examined the potential of hepatitis B virus (HBV) vaccines based on mouse CMV (MCMV) vectors expressing the small HBsAg. Immunological consequences of vaccine virus attenuation were addressed by either replacing the dispensable gene\n                    <jats:italic>m157<\/jats:italic>\n                    (\u201cMCMV\u2010HBs\u020d) or the gene\n                    <jats:italic>M27<\/jats:italic>\n                    (\u201c\u0394M27\u2010HBs\u201d), the latter encodes a potent IFN antagonist targeting the transcription factor STAT2.\n                    <jats:italic>M27<\/jats:italic>\n                    was chosen, since human CMV encodes an analogous gene product, which also induced proteasomal STAT2 degradation by exploiting Cullin RING ubiquitin ligases. Vaccinated mice were challenged with HBV through hydrodynamic injection. MCMV\u2010HBs and \u0394M27\u2010HBs vaccination achieved accelerated HBV clearance in serum and liver as well as robust HBV\u2010specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell responses. When we explored the therapeutic potential of MCMV\u2010based vaccines, especially the combination of \u0394M27\u2010HBs prime and DNA boost vaccination resulted in increased intrahepatic HBs\u2010specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell responses and HBV clearance in persistently infected mice. Our results demonstrated that vaccines based on a replication competent MCMV attenuated through the deletion of an IFN antagonist targeting STAT2 elicit robust anti\u2010HBV immune responses and mediate HBV clearance in mice in prophylactic and therapeutic immunization regimes.\n                  <\/jats:p>","DOI":"10.1002\/eji.202048780","type":"journal-article","created":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T01:31:08Z","timestamp":1602120668000},"page":"393-407","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Prophylactic and therapeutic HBV vaccination by an HBs\u2010expressing cytomegalovirus vector lacking an interferon antagonist in mice"],"prefix":"10.1002","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7720-4277","authenticated-orcid":false,"given":"Hongming","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meike","family":"R\u00fcckborn","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital of Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vu Thuy Khanh","family":"Le\u2010Trilling","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital of Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shangqing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenqing","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuecheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemei","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinping","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengji","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital of Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulf","family":"Dittmer","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital of Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongliang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirko","family":"Trilling","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital of Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8262-4997","authenticated-orcid":false,"given":"Jia","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)61412-X"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0801644"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2012.02.010"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-014-3410-5"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.1.68-76.2003"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-8278(91)90074-L"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1573"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2018.04.003"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(01)00028-9"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.09.072"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002565"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.11.019"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2013.05.003"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2017.03.011"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2014-308041"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.12.060"},{"key":"e_1_2_9_18_1","first-page":"93","article-title":"A highly attenuated vesicular stomatitis virus\u2010based vaccine platform controls hepatitis B virus replication in mouse models of hepatitis B","author":"Moshkani S.","year":"2019","journal-title":"J. Virol."},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines7030062"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.03.029"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4473"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10003"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1237874"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-019-00597-7"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1935"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02978"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002069"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20071636"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00296-18"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.01.070"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.14.7536-7544.2004"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041401"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.4.1697-1707.2001"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)34034-1"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0282"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302523"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.4.2630-2637.1998"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101612"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-55508-w"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608578103"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1086\/323993"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0913498107"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050882"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-002-8488-5"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.4.2022"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.5.2255-2264.2004"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.07.017"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI42758"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00299-16"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)70487-6"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.1.185"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.10.8612-8622.1999"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-01-069112"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01177-06"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02851-13"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02474-07"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012926"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00054"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-5-47"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01139"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.8.4465"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.2144\/000112096"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-652-8_30"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00545-11"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0174695"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02042-08"},{"key":"e_1_2_9_67_1","doi-asserted-by":"crossref","first-page":"e51","DOI":"10.1002\/cpim.51","article-title":"Cytomegalovirus infection: mouse model","author":"Brizic I.","year":"2018","journal-title":"Curr. Protoc. Immunol."},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00219"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00996-14"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608578103"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24195"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/281646a0"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2012.03.006"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0151717"},{"key":"e_1_2_9_75_1","article-title":"Isolation of mouse intrahepatic lymphocytes","author":"Crispe I. N.","year":"2001","journal-title":"Curr. Protoc. Immunol."},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201970107"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.202048780","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/eji.202048780","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.202048780","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T04:06:24Z","timestamp":1696824384000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.202048780"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,9]]},"references-count":75,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/eji.202048780"],"URL":"https:\/\/doi.org\/10.1002\/eji.202048780","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.01.29.924787","asserted-by":"object"}],"has-review":[{"id-type":"doi","id":"10.1002\/EJI.202048780\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202048780\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202048780\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202048780\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202048780\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202048780\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202048780\/v2\/review1","asserted-by":"object"}]},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,9]]},"assertion":[{"value":"2020-05-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}