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This study was designed to verify whether loss of its function might alleviate inflammatory events in joints of experimental and rheumatoid arthritis (RA). Dark Agouti (DA) rats were injected intradermally with pristane to induce arthritis, and rno\u2010miR\u2010147 antagomir was locally administrated into individual ankle compared with negative control or rno\u2010miR\u2010155\u20105p antagomir (potential positive control). Arthritis onset, macroscopic severity, and pathological changes were monitored. While in vitro, gain or loss function of hsa\u2010miR\u2010147b\u20103p\/hsa\u2010miR\u2010155\u20105p and ZNF148 was achieved in human synovial fibroblast cell line SW982 and RA synovial fibroblasts (RASF). The expression of miRNAs and mRNAs was detected by using RT\u2010quantitative PCR, and protein expression was detected by using Western blotting. Anti\u2010miR\u2010147 therapy could alleviate the severity, especially for the synovitis and joint destruction in experimental arthritis. Gain of hsa\u2010miR\u2010147b\u20103p\/hsa\u2010miR\u2010155\u20105p function in TNF\u2010\u03b1 stimulated SW982 and RASF cells could upregulate, in contrast, loss of hsa\u2010miR\u2010147b\u20103p\/hsa\u2010miR\u2010155\u20105p function could downregulate the gene expression of\n                    <jats:italic>TNF\u2010\u03b1, IL\u20106, MMP3<\/jats:italic>\n                    , and\n                    <jats:italic>MMP13<\/jats:italic>\n                    . Hence, such alteration could participate in synovial inflammation and joint destruction. RNAi of ZNF148, a miR\u2010147's target, increased gene expression of\n                    <jats:italic>TNF\u2010\u03b1, IL\u20106, MMP3<\/jats:italic>\n                    , and\n                    <jats:italic>MMP13<\/jats:italic>\n                    in SW982 and RASF cells. Also, mRNA sequencing data showed that hsa\u2010miR\u2010147b\u20103p mimic and ZNF148 siRNA commonly regulated the gene expression of\n                    <jats:italic>CCL3<\/jats:italic>\n                    and\n                    <jats:italic>DEPTOR<\/jats:italic>\n                    as well as some arthritis and inflammation\u2010related pathways. Taken together, miR\u2010147b\u20103p contributes to synovial inflammation through repressing ZNF148 in RA and experimental arthritis.\n                  <\/jats:p>","DOI":"10.1002\/eji.202048850","type":"journal-article","created":{"date-parts":[[2021,4,17]],"date-time":"2021-04-17T05:17:25Z","timestamp":1618636645000},"page":"2062-2073","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Loss of microRNA\u2010147 function alleviates synovial inflammation through ZNF148 in rheumatoid and experimental arthritis"],"prefix":"10.1002","volume":"51","author":[{"given":"Congshan","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Institute of Molecular and Translational Medicine (IMTM), School of Basic Medical Sciences Xi'an Jiaotong University Health Science Center  Xi'an Shaanxi P. R. 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