{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:49:19Z","timestamp":1790232559562,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T00:00:00Z","timestamp":1614038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R37AI39560"],"award-info":[{"award-number":["R37AI39560"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32 GM113846"],"award-info":[{"award-number":["T32 GM113846"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    TCR\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    CD8\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    CD8\u03b2\n                    <jats:sup>\u2013<\/jats:sup>\n                    intestinal intraepithelial lymphocytes (CD8\u03b1\u03b1 IEL) are gut T cells that maintain barrier surface homeostasis. Most CD8\u03b1\u03b1 IEL are derived from thymic precursors (IELp) through a mechanism referred to as clonal diversion. In this model, self\u2010reactive thymocytes undergo deletion in the presence of CD28 costimulation, but in its absence undergo diversion to the IEL fate. While previous reports showed that IELp were largely \u03b22m dependent, the APC that drive the development of these cells are poorly defined. We found that both CD80 and CD86 restrain IELp development, and conventional DCs play a prominent role. We sought to define a CD80\/86 negative, MHCI positive APC that supports the development to the IEL lineage. Chimera studies showed that MHCI needs to be expressed on hematopoietic APC for selection. As thymic hematopoietic APC are heterogeneous in their expression of MHCI and costimulatory molecules, we identified four thymic APC types that were CD80\/86\n                    <jats:sup>neg\/low<\/jats:sup>\n                    and MHCI\n                    <jats:sup>+<\/jats:sup>\n                    . However, selective depletion of \u03b22m in individual APC suggested functional redundancy. Thus, while hematopoietic APC play a critical role in clonal diversion, no single APC subset is specialized to promote the CD8\u03b1\u03b1 IEL fate.\n                  <\/jats:p>","DOI":"10.1002\/eji.202048996","type":"journal-article","created":{"date-parts":[[2021,1,31]],"date-time":"2021-01-31T21:59:30Z","timestamp":1612130370000},"page":"1080-1088","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["MHC Class I on murine hematopoietic APC selects Type A IEL precursors in the thymus"],"prefix":"10.1002","volume":"51","author":[{"given":"S. Thera","family":"Lee","sequence":"first","affiliation":[{"name":"Center for Immunology and Department of Laboratory Medicine &amp; Pathology University of Minnesota  Minneapolis MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1369-960X","authenticated-orcid":false,"given":"Hristo","family":"Georgiev","sequence":"additional","affiliation":[{"name":"Center for Immunology and Department of Laboratory Medicine &amp; Pathology University of Minnesota  Minneapolis MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise R.","family":"Breed","sequence":"additional","affiliation":[{"name":"Center for Immunology and Department of Laboratory Medicine &amp; Pathology University of Minnesota  Minneapolis MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Ruscher","sequence":"additional","affiliation":[{"name":"Center for Immunology and Department of Laboratory Medicine &amp; Pathology University of Minnesota  Minneapolis MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9963-5687","authenticated-orcid":false,"given":"Kristin A.","family":"Hogquist","sequence":"additional","affiliation":[{"name":"Center for Immunology and Department of Laboratory Medicine &amp; Pathology University of Minnesota  Minneapolis MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11894-010-0148-6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3007"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00284-4"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-018-0013-7"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.08.018"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.07.010"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.07.008"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20170844"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3751"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2017.54"},{"key":"e_1_2_8_12_1","first-page":"217","article-title":"Intestinal CD8alphaalpha IEL derived from two distinct thymic precursors have staggered ontogeny","author":"Ruscher R.","year":"2020","journal-title":"J. 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