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Within the GC microenvironment, GC B cells undergo proliferation and B cell receptor (BCR) genes somatic hypermutation in the dark zone (DZ), and affinity\u2010based selection in the light zone (LZ). In the current paradigm of GC dynamics, high\u2010affinity LZ B cells may be selected by cognate T\u2010 follicular helper cells to either differentiate into plasma cells or memory B cells, or re\u2010enter the DZ and initiate a new round of proliferation and BCR diversification, before migrating back to the LZ. Given the diversity of cell states and potential cell fates that GC B cells may adopt, the two\u2010state DZ\u2010LZ paradigm has been challenged by studies that explored GC B\u2010cell heterogeneity with a variety of single\u2010cell technologies. Here, we review studies and single\u2010cell technologies which have allowed to refine the working model of GC B\u2010cell cellular and molecular heterogeneity during affinity maturation. This review also covers the use of single\u2010cell quantitative data for mathematical modeling of GC reactions, and the application of single\u2010cell genomics to the study of GC\u2010derived malignancies.<\/jats:p>","DOI":"10.1002\/eji.202149235","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T14:42:03Z","timestamp":1627569723000},"page":"2555-2567","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Heterogeneity of germinal center B cells: New insights from single\u2010cell studies"],"prefix":"10.1002","volume":"51","author":[{"given":"Noudjoud","family":"Attaf","sequence":"first","affiliation":[{"name":"Aix Marseille Universit\u00e9 CNRS INSERM Centre d'Immunologie de Marseille\u2010Luminy (CIML)  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Baaklini","sequence":"additional","affiliation":[{"name":"Aix Marseille 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