{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:08:36Z","timestamp":1772118516951,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T00:00:00Z","timestamp":1631491200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Both B cells and T cells are involved in an effective immune response to SARS\u2010CoV\u20102, the disease\u2010causing virus of COVID\u201019. While B cells\u2014with the indispensable help of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells\u2014are essential to generate neutralizing antibodies, T cells on their own have been recognized as another major player in effective anti\u2010SARS\u2010CoV\u20102 immunity. In this report, we provide insights into the characteristics of individual HLA\u2010A*02:01\u2010 and HLA\u2010A*24:02\u2010restricted SARS\u2010CoV\u20102\u2010reactive TCRs, isolated from convalescent COVID\u201019 patients. We observed that SARS\u2010CoV\u20102\u2010reactive T\u2010cell populations were clearly detectable in convalescent samples and that TCRs isolated from these T cell clones were highly functional upon ectopic re\u2010expression. The SARS\u2010CoV\u20102\u2010reactive TCRs described in this report mediated potent TCR signaling in reporter assays with low nanomolar EC50 values. We further demonstrate that these SARS\u2010CoV\u20102\u2010reactive TCRs conferred powerful T\u2010cell effector function to primary CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells as evident by a robust anti\u2010SARS\u2010CoV\u20102 IFN\u2010\u03b3 response and in vitro cytotoxicity. We also provide an example of a long\u2010lasting anti\u2010SARS\u2010CoV\u20102 memory response by reisolation of one of the retrieved TCRs 5 months after initial sampling. Taken together, these findings contribute to a better understanding of anti\u2010SARS\u2010CoV\u20102 T\u2010cell immunity and may contribute to paving the way toward immunotherapeutics approaches targeting SARS\u2010CoV\u20102.\n                  <\/jats:p>","DOI":"10.1002\/eji.202149290","type":"journal-article","created":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T16:10:16Z","timestamp":1629735016000},"page":"2651-2664","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["SARS\u2010CoV\u20102\u2010reactive T\u2010cell receptors isolated from convalescent COVID\u201019 patients confer potent T\u2010cell effector function"],"prefix":"10.1002","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9667-7435","authenticated-orcid":false,"given":"Fabian","family":"Brunk","sequence":"first","affiliation":[{"name":"Immatics N.V.  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K.","family":"Fraschka","sequence":"additional","affiliation":[{"name":"NGS Competence Center T\u00fcbingen  T\u00fcbingen Germany"},{"name":"Institute of Medical Genetics and Applied Genomics University Hospital T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7305-8620","authenticated-orcid":false,"given":"Jonas S.","family":"Heitmann","sequence":"additional","affiliation":[{"name":"Clinical Collaboration Unit Translational Immunology German Cancer Consortium (DKTK) Department of Internal Medicine University Hospital T\u00fcbingen  T\u00fcbingen Germany"},{"name":"Cluster of Excellence iFIT (EXC2180) \u2018Image\u2010Guided and Functionally Instructed Tumor Therapies,\u2019 University of T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5718-1530","authenticated-orcid":false,"given":"Sebastian","family":"H\u00f6rber","sequence":"additional","affiliation":[{"name":"Department for Diagnostic Laboratory Medicine Institute for Clinical Chemistry and Pathobiochemistry University Hospital T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Peter","sequence":"additional","affiliation":[{"name":"Department for Diagnostic Laboratory Medicine Institute for Clinical Chemistry and Pathobiochemistry University Hospital T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1614-2647","authenticated-orcid":false,"given":"Hans\u2010Georg","family":"Rammensee","sequence":"additional","affiliation":[{"name":"Department of Immunology Institute for Cell Biology University of T\u00fcbingen  T\u00fcbingen Germany"},{"name":"Cluster of Excellence iFIT (EXC2180) \u2018Image\u2010Guided and Functionally Instructed Tumor Therapies,\u2019 University of T\u00fcbingen  T\u00fcbingen Germany"},{"name":"German Cancer Consortium (DKTK) and German Cancer Research Center (DKFZ) Partner Site T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harpreet","family":"Singh","sequence":"additional","affiliation":[{"name":"Immatics N.V.  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