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Here, we report the development of a quick and inexpensive\n                    <jats:italic>su<\/jats:italic>\n                    rrogate SARS\u2010CoV\u20102\n                    <jats:italic>b<\/jats:italic>\n                    locking\n                    <jats:italic>a<\/jats:italic>\n                    ssay (SUBA) using immobilized recombinant human angiotensin\u2010converting enzyme 2 (hACE2) and human cells expressing the native form of surface SARS\u2010CoV\u20102 spike protein. Spike protein\u2010expressing cells bound to hACE2 in the absence or presence of blocking antibodies were quantified by measuring the optical density of cell\u2010associated crystal violet in a spectrophotometer. The advantages are that SUBA is a fast and inexpensive assay, which does not require biosafety level 2\u2010 or 3\u2010approved laboratories. Most importantly, SUBA detects blocking antibodies against the native trimeric cell\u2010bound SARS\u2010CoV\u20102 spike protein and can be rapidly adjusted to quickly pre\u2010screen already approved therapeutic antibodies or sera from vaccinated individuals for their ACE2 blocking activities against any emerging SARS\u2010CoV\u20102 variants.\n                  <\/jats:p>","DOI":"10.1002\/eji.202149302","type":"journal-article","created":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T17:15:54Z","timestamp":1632244554000},"page":"2665-2676","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A surrogate cell\u2010based SARS\u2010CoV\u20102 spike blocking assay"],"prefix":"10.1002","volume":"51","author":[{"given":"Wolfgang","family":"Schuh","sequence":"first","affiliation":[{"name":"Division of Molecular Immunology, Department of Internal Medicine 3, Nikolaus\u2010Fiebiger\u2010Zentrum Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lena","family":"Baus","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology, Department of Internal Medicine 3, Nikolaus\u2010Fiebiger\u2010Zentrum Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobit","family":"Steinmetz","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology, Department of Internal Medicine 3, Nikolaus\u2010Fiebiger\u2010Zentrum Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian R.","family":"Schulz","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology, Department of Internal Medicine 3, Nikolaus\u2010Fiebiger\u2010Zentrum Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonie","family":"Weckwerth","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology, Department of Internal Medicine 3, Nikolaus\u2010Fiebiger\u2010Zentrum Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edith","family":"Roth","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology, Department of Internal Medicine 3, Nikolaus\u2010Fiebiger\u2010Zentrum Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Hauke","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology, Department of Internal Medicine 3, Nikolaus\u2010Fiebiger\u2010Zentrum Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Krause","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology, Department of Internal Medicine 3, Nikolaus\u2010Fiebiger\u2010Zentrum Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Morhart","sequence":"additional","affiliation":[{"name":"Division of Molecular Pediatrics, Department of Pediatrics Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred","family":"Rauh","sequence":"additional","affiliation":[{"name":"Division of Molecular Pediatrics, Department of 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