{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:06:55Z","timestamp":1772118415381,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T00:00:00Z","timestamp":1636934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19H03682"],"award-info":[{"award-number":["19H03682"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["20ek0109400h0003"],"award-info":[{"award-number":["20ek0109400h0003"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Multiple embryonic precursors give rise to leukocytes in adults while the lineage\u2010based functional impacts are underappreciated. Mesodermal precursors expressing PDGFR\u03b1 appear transiently during E7.5\u20108.5 descend to a subset of Lin\n                    <jats:sup>\u2013<\/jats:sup>\n                    Sca1\n                    <jats:sup>+<\/jats:sup>\n                    Kit\n                    <jats:sup>+<\/jats:sup>\n                    hematopoietic progenitors found in adult BM. By analyzing a PDGFR\u03b1\u2010lineage tracing mouse line, we here report that PDGFR\u03b1\u2010lineage BM F4\/80\n                    <jats:sup>+<\/jats:sup>\n                    SSC\n                    <jats:sup>lo<\/jats:sup>\n                    monocytes\/macrophages are solely Ly6C\n                    <jats:sup>+<\/jats:sup>\n                    LFA\u20101\n                    <jats:sup>hi<\/jats:sup>\n                    Mac\u20101\n                    <jats:sup>hi<\/jats:sup>\n                    monocytes enriched on the abluminal sinusoidal endothelium while Ly6C\n                    <jats:sup>\u2013<\/jats:sup>\n                    LFA\u20101\n                    <jats:sup>lo<\/jats:sup>\n                    Mac\u20101\n                    <jats:sup>lo<\/jats:sup>\n                    macrophages are mostly from non\u2010PDGFR\u03b1\u2010lineage in vivo. Monocytes with stronger integrin profiles outcompete macrophages for adhesion on an endothelial monolayer or surfaces coated with ICAM\u20101\u2010Fc or VCAM\u20101\u2010Fc. Egress of PDGFR\u03b1\u2010lineage\u2010rich monocytes and subsequent differentiation to peripheral macrophages spatially segregates them from non\u2010PDGFR\u03b1\u2010lineage BM\u2010resident macrophages and allows functional specialization since macrophages derived from these egressing monocytes differ in morphology, phenotype, and functionality from BM\u2010resident macrophages in culture. Extravasation preference for blood PDGFR\u03b1\u2010lineage monocytes varies by tissues and governs the local lineage composition of macrophages. More PDGFR\u03b1\u2010lineage classical monocytes infiltrated into skin and colon but not into peritoneum. Accordingly, transcriptomic analytics indicated augmented inflammatory cascades in dermatitis skin of BM\u2010chimeric mice harbouring only PDGFR\u03b1\u2010lineage leukocytes. Thus, the PDGFR\u03b1\u2010lineage origin biasedly generates monocytes predestined for BM exit to support peripheral immunity following extravasation and macrophage differentiation.\n                  <\/jats:p>","DOI":"10.1002\/eji.202149479","type":"journal-article","created":{"date-parts":[[2021,10,28]],"date-time":"2021-10-28T05:56:40Z","timestamp":1635400600000},"page":"204-221","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["PDGFR\u03b1\u2010lineage origin directs monocytes to trafficking proficiency to support peripheral immunity"],"prefix":"10.1002","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0718-7344","authenticated-orcid":false,"given":"Yu\u2010Tung","family":"Li","sequence":"first","affiliation":[{"name":"Department of Stem Cell Therapy Science Graduate School of Medicine Osaka University  Suita Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sho","family":"Yamazaki","sequence":"additional","affiliation":[{"name":"StemRIM Inc.  Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiichi","family":"Takaki","sequence":"additional","affiliation":[{"name":"StemRIM Inc.  Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuya","family":"Ouchi","sequence":"additional","affiliation":[{"name":"StemRIM Inc.  Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomomi","family":"Kitayama","sequence":"additional","affiliation":[{"name":"StemRIM Inc.  Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuto","family":"Tamai","sequence":"additional","affiliation":[{"name":"Department of Stem Cell Therapy Science Graduate School of Medicine Osaka University  Suita Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.10.008"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-07-343566"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.79.6.1196"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-12-259556"},{"key":"e_1_2_10_6_1","doi-asserted-by":"crossref","unstructured":"Li Yu\u2010T Goswami D. Follmer M. Artz A. Pacheco\u2010Blanco M. 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