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Therefore, we characterized the cellularity, surface marker expression, and iron transporter phenotype of neutrophils and monocyte subsets in COVID\u201019 patients within 72 h from hospital admission, and analyzed how these parameters relate to infection severity. Between March and November 2020, blood leukocyte samples from hospitalized COVID\u201019 patients (n = 48) and healthy individuals (n = 7) were analyzed by flow cytometry enabling comparative analysis of 40 features. Inflammation\u2010driven neutrophil expansion, depletion of CD16<jats:sup>+<\/jats:sup> nonclassical monocytes, and changes in surface expression of neutrophil and monocyte CD64 and CD86 were associated with COVID\u201019 severity. By unsupervised self\u2010organizing map clustering, four patterns of innate myeloid response were identified and linked to varying levels of systemic inflammation, altered cellular iron trafficking and the severity of disease. These alterations of the myeloid leukocyte compartment during acute COVID\u201019 may be hallmarks of inefficient viral control and immune hyperactivation and may help at risk prediction and treatment optimization.<\/jats:p>","DOI":"10.1002\/eji.202149680","type":"journal-article","created":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T08:21:45Z","timestamp":1651479705000},"page":"1285-1296","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Alterations of blood monocyte subset distribution and surface phenotype are linked to infection severity in COVID\u201019 inpatients"],"prefix":"10.1002","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8028-1030","authenticated-orcid":false,"given":"David","family":"Haschka","sequence":"first","affiliation":[{"name":"Department of Internal Medicine II Medical University of Innsbruck  Innsbruck 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