{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:39:28Z","timestamp":1786977568546,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T00:00:00Z","timestamp":1676419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007109","name":"Universit\u00e0 degli Studi di Ferrara","doi-asserted-by":"publisher","award":["2020\u2010FAR.L\u2010CF_003"],"award-info":[{"award-number":["2020\u2010FAR.L\u2010CF_003"]}],"id":[{"id":"10.13039\/501100007109","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007109","name":"Universit\u00e0 degli Studi di Ferrara","doi-asserted-by":"publisher","award":["2021\u2010FAR.L\u2010CF_002"],"award-info":[{"award-number":["2021\u2010FAR.L\u2010CF_002"]}],"id":[{"id":"10.13039\/501100007109","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Psoriasis is a chronic inflammatory skin disease with an autoimmune component and associated with joint inflammation in up to 30% of cases. To investigate autoreactive T cells, we developed an imiquimod\u2010induced psoriasis\u2010like inflammation model in K5\u2010mOVA.tg C57BL\/6 mice expressing ovalbumin (OVA) on the keratinocyte membrane, adoptively transferred with OT\u2010I OVA\u2010specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u00a0cells. We evaluated the expansion of OT\u2010I CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells and their localization in skin, blood, and spleen. scRNA\u2010seq and TCR sequencing data from patients with psoriatic arthritis were also analyzed. In the imiquimod\u2010treated K5\u2010mOVA.tg mouse model, OT\u2010I T cells were markedly expanded in the skin and blood at early time points. OT\u2010I T cells in the skin showed mainly CXCR3\n                    <jats:sup>+<\/jats:sup>\n                    effector memory phenotype, whereas in peripheral blood there was an expansion of CCR4\n                    <jats:sup>+<\/jats:sup>\n                    CXCR3\n                    <jats:sup>+<\/jats:sup>\n                    OT\u2010I cells. At a later time point, expanded OVA\u2010specific T\u2010cell population was found in the spleen. In patients with psoriatic arthritis, scRNA\u2010seq and TCR sequencing data showed clonal expansion of CCR4\n                    <jats:sup>+<\/jats:sup>\n                    T\n                    <jats:sub>CM<\/jats:sub>\n                    cells in the circulation and further expansion in the synovial fluid. Importantly, there was a clonotype overlap between CCR4\n                    <jats:sup>+<\/jats:sup>\n                    T\n                    <jats:sub>CM<\/jats:sub>\n                    in the peripheral blood and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell effectors in the synovial fluid. This mechanism could play a role in the generation and spreading of autoreactive T cells to the synovioentheseal tissues in psoriasis patients at risk of developing psoriatic arthritis.\n                  <\/jats:p>","DOI":"10.1002\/eji.202149702","type":"journal-article","created":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T07:14:36Z","timestamp":1675235676000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["CCR4\n                    <sup>+<\/sup>\n                    CD8\n                    <sup>+<\/sup>\n                    T cells clonally expand to differentiated effectors in murine psoriasis and in human psoriatic arthritis"],"prefix":"10.1002","volume":"53","author":[{"given":"Guendalina","family":"Montico","sequence":"first","affiliation":[{"name":"IRCCS Istituto Ortopedico Galeazzi  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Mingozzi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biosciences University of Milano\u2010Bicocca  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6431-3335","authenticated-orcid":false,"given":"Fabio","family":"Casciano","sequence":"additional","affiliation":[{"name":"Department of Translational Medicine and LTTA Centre University of Ferrara  Ferrara Italy"},{"name":"Interdepartmental Research Center for the Study of Multiple Sclerosis and Inflammatory and Degenerative Diseases of the Nervous System University of Ferrara  Ferrara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Protti","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biosciences University of Milano\u2010Bicocca  Milan Italy"},{"name":"National Institute of Molecular Genetics \u201cRomeo ed Enrica Invernizzi\u201d (INGM)  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Gornati","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biosciences University of Milano\u2010Bicocca  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Marzola","sequence":"additional","affiliation":[{"name":"Department of Chemical Pharmaceutical and Agricultural Sciences University of Ferrara  Ferrara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Banfi","sequence":"additional","affiliation":[{"name":"IRCCS Istituto Ortopedico Galeazzi  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remo","family":"Guerrini","sequence":"additional","affiliation":[{"name":"Department of Chemical Pharmaceutical and Agricultural Sciences University of Ferrara  Ferrara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Secchiero","sequence":"additional","affiliation":[{"name":"Department of Translational Medicine and LTTA Centre University of Ferrara  Ferrara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Volinia","sequence":"additional","affiliation":[{"name":"Department of Translational Medicine and LTTA Centre University of Ferrara  Ferrara Italy"},{"name":"Biological and Chemical Research Centre (CNBCh UW) University of Warsaw  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7046-4914","authenticated-orcid":false,"given":"Francesca","family":"Granucci","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biosciences University of Milano\u2010Bicocca  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1900-1356","authenticated-orcid":false,"given":"Eva","family":"Reali","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biosciences University of Milano\u2010Bicocca  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,15]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)61909-7"},{"key":"e_1_2_12_3_1","first-page":"S132","article-title":"Use of methotrexate in patients with psoriatic arthritis","volume":"28","author":"Ceponis A.","year":"2010","journal-title":"Clin. Exp. Rheumatol."},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-010-0107-0"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-012-8308-0"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)00184-7"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02466-4"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02746"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00200"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201404114"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.04.021"},{"key":"e_1_2_12_12_1","doi-asserted-by":"crossref","DOI":"10.1111\/sji.12945","article-title":"Current knowledge on autoantigens and autoantibodies in psoriasis","volume":"92","author":"Bergen L. L. T.","year":"2020","journal-title":"Scand. J. Immunol."},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00954"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.20643"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20151093"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6621"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2016.03.019"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202805"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-47310-5"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2017.04.001"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18513-6"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0802999"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200323630"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1508990112"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2013.03.006"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302313"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.08.048"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1014154108"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00529"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-015-1835-0"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.06.014"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.09.013"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032712-095954"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aav8995"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2020.12.034"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/ced.12251"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12980-2"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02191"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21197228"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.3.1935"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.11.6123"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri724"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.048"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1874-1"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0619-0"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0276-y"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2013.094243"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11247"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.22139.1"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.202149702","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/eji.202149702","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.202149702","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T01:07:55Z","timestamp":1723770475000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.202149702"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,15]]},"references-count":48,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["10.1002\/eji.202149702"],"URL":"https:\/\/doi.org\/10.1002\/eji.202149702","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/EJI.202149702\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202149702\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202149702\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202149702\/v4\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202149702\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202149702\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202149702\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202149702\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202149702\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202149702\/v4\/response1","asserted-by":"object"}]},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,15]]},"assertion":[{"value":"2021-10-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2149702"}}