{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:41:42Z","timestamp":1786977702566,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T00:00:00Z","timestamp":1675382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Thymic epithelial cells (TECs) are key effectors of the thymic stroma and are critically required for T\u2010cell development. TECs comprise a diverse set of related but functionally distinct cell types that are scarce and difficult to isolate and handle. This has precluded TEC\u2010based screening assays. We previously described induced thymic epithelial cells (iTECs), an artificial cell type produced\n                    <jats:italic>in vitro<\/jats:italic>\n                    by direct reprogramming, raising the possibility that iTECs might provide the basis for functional screens related to TEC biology. Here, we present an iTEC\u2010based three\u2010stage medium\/high\u2010throughput\n                    <jats:italic>in vitro<\/jats:italic>\n                    assay for synthetic polymer mimics of thymic extracellular matrix (ECM). Using this assay, we identified, from a complex library, four polymers that bind iTEC as well as or better than gelatin but do not bind mesenchymal cells. We show that these four polymers also bind and maintain native mouse fetal TECs and native human fetal TECs. Finally, we show that the selected polymers do not interfere with iTEC function or T\u2010cell development. Collectively, our data establish that iTECs can be used to screen for TEC\u2010relevant compounds in at least some medium\/high\u2010throughput assays and identify synthetic polymer ECM mimics that can replace gelatin or ECM components in TEC culture protocols.\n                  <\/jats:p>","DOI":"10.1002\/eji.202249934","type":"journal-article","created":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T08:05:36Z","timestamp":1673856336000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["An induced thymic epithelial cell\u2010based high throughput screen for thymus extracellular matrix mimetics"],"prefix":"10.1002","volume":"53","author":[{"given":"Paul","family":"Rouse","sequence":"first","affiliation":[{"name":"Centre for Regenerative Medicine School of Biological Sciences Institute for Stem Cell Research  5 Little France Drive Edinburgh EH16 4UU UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Henderson","sequence":"additional","affiliation":[{"name":"Centre for Regenerative Medicine School of Biological Sciences Institute for Stem Cell Research  5 Little France Drive Edinburgh EH16 4UU UK"},{"name":"Australian National University Medical School The Canberra Hospital  Garran ACT 2605 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seshasailam","family":"Venkateswaran","sequence":"additional","affiliation":[{"name":"School of Chemistry University of Edinburgh  Joseph Black Building Edinburgh EH8 9YL UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Sweetman","sequence":"additional","affiliation":[{"name":"Centre for Regenerative Medicine School of Biological Sciences Institute for Stem Cell Research  5 Little France Drive Edinburgh EH16 4UU UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cairnan","family":"Duffy","sequence":"additional","affiliation":[{"name":"School of Chemistry University of Edinburgh  Joseph Black Building Edinburgh EH8 9YL UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Bradley","sequence":"additional","affiliation":[{"name":"School of Chemistry University of Edinburgh  Joseph Black Building Edinburgh EH8 9YL UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Clare","family":"Blackburn","sequence":"additional","affiliation":[{"name":"Centre for Regenerative Medicine School of Biological Sciences Institute for Stem Cell Research  5 Little France Drive Edinburgh EH16 4UU UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-051116-052320"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/362070a0"},{"key":"e_1_2_10_4_1","first-page":"51","article-title":"T\u2010cell differentiation in thymus organ cultures","volume":"2","author":"Jenkinson E. J.","year":"1990","journal-title":"Semin. 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