{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:20:35Z","timestamp":1790720435856,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T00:00:00Z","timestamp":1667520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004807","name":"California Department of Fish and Game","doi-asserted-by":"publisher","award":["GRK1949"],"award-info":[{"award-number":["GRK1949"]}],"id":[{"id":"10.13039\/100004807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004807","name":"California Department of Fish and Game","doi-asserted-by":"publisher","award":["GRK 2098"],"award-info":[{"award-number":["GRK 2098"]}],"id":[{"id":"10.13039\/100004807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004807","name":"California Department of Fish and Game","doi-asserted-by":"publisher","award":["WE4472\/8\u20101"],"award-info":[{"award-number":["WE4472\/8\u20101"]}],"id":[{"id":"10.13039\/100004807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004807","name":"California Department of Fish and Game","doi-asserted-by":"publisher","award":["TR1208\/1\u20101"],"award-info":[{"award-number":["TR1208\/1\u20101"]}],"id":[{"id":"10.13039\/100004807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004807","name":"California Department of Fish and Game","doi-asserted-by":"publisher","award":["TR1208\/2\u20101"],"award-info":[{"award-number":["TR1208\/2\u20101"]}],"id":[{"id":"10.13039\/100004807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017222","name":"Kulturstiftung Essen","doi-asserted-by":"publisher","award":["106\u201022078"],"award-info":[{"award-number":["106\u201022078"]}],"id":[{"id":"10.13039\/100017222","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017222","name":"Kulturstiftung Essen","doi-asserted-by":"publisher","award":["106\u201021590"],"award-info":[{"award-number":["106\u201021590"]}],"id":[{"id":"10.13039\/100017222","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010380","name":"Stiftung Universit\u00e4tsmedizin Essen","doi-asserted-by":"publisher","award":["2020 4699 116"],"award-info":[{"award-number":["2020 4699 116"]}],"id":[{"id":"10.13039\/501100010380","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Primary and recurrent cytomegalovirus (CMV) infections frequently cause CMV colitis in immunocompromised as well as inflammatory bowel disease (IBD) patients. Additionally, colitis occasionally occurs upon primary CMV infection in patients who are apparently immunocompetent. In both cases, the underlying pathophysiologic mechanisms are largely elusive \u2010 in part due to the lack of adequate access to specimens. We employed the mouse cytomegalovirus (MCMV) model to assess the association between CMV and colitis. During acute primary MCMV infection of immunocompetent mice, the gut microbial composition was affected as manifested by an altered ratio of the\n                    <jats:italic>Firmicutes<\/jats:italic>\n                    to\n                    <jats:italic>Bacteroidetes<\/jats:italic>\n                    phyla. Interestingly, these microbial changes coincided with high\u2010titer MCMV replication in the colon, crypt hyperplasia, increased colonic pro\u2010inflammatory cytokine levels, and a transient increase in the expression of the antimicrobial protein\n                    <jats:italic>Regenerating islet\u2010derived protein 3 gamma<\/jats:italic>\n                    (\n                    <jats:italic>Reg3\u03b3<\/jats:italic>\n                    ). Further analyses revealed that murine and human intestinal epithelial cell lines, as well as primary intestinal crypt cells and organoids represent direct targets of CMV infection causing increased cell death. Accordingly,\n                    <jats:italic>in vivo<\/jats:italic>\n                    MCMV infection disrupted the intestinal epithelial barrier and increased apoptosis of intestinal epithelial cells. In summary, our data show that CMV transiently induces colitis in immunocompetent hosts by altering the intestinal homeostasis.\n                  <\/jats:p>","DOI":"10.1002\/eji.202249940","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T05:33:40Z","timestamp":1665984820000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Acute cytomegalovirus infection modulates the intestinal microbiota and targets intestinal epithelial cells"],"prefix":"10.1002","volume":"53","author":[{"given":"Vu Thuy Khanh","family":"Le\u2010Trilling","sequence":"first","affiliation":[{"name":"Institute for Virology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana\u2010Fabienne","family":"Ebel","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Baier","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Wohlgemuth","sequence":"additional","affiliation":[{"name":"Institute for Virology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai Robin","family":"Pfeifer","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aart","family":"Mookhoek","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4918-6654","authenticated-orcid":false,"given":"Philippe","family":"Krebs","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madita","family":"Determann","sequence":"additional","affiliation":[{"name":"Institute for Virology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Katschinski","sequence":"additional","affiliation":[{"name":"Institute for Virology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Adamczyk","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Lange","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Klopfleisch","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Pathology Free University of Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian M.","family":"Lange","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktoriya","family":"Sokolova","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"},{"name":"Inorganic Chemistry and Centre for Nanointegration Duisburg\u2010Essen (CeNIDE) University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirko","family":"Trilling","sequence":"additional","affiliation":[{"name":"Institute for Virology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid M.","family":"Westendorf","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-5-47"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.5217\/ir.2018.16.3.504"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365549850161098"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/000313480707300113"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2013.12.004"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2013.00034"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00045.2009"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2015.186"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mib.0000231576.11678.57"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v12.i30.4813"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365520310000546"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-020-0332-7"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.42"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2013.116"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.02.005"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/COH.0000000000000234"},{"key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"e00167","DOI":"10.1128\/JVI.00167-18","article-title":"Subclinical Cytomegalovirus Infection Is Associated with Altered Host Immunity, Gut Microbiota, and Vaccine Responses","volume":"92","author":"Santos Rocha C","year":"2018","journal-title":"J. Virol."},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706625104"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph13110346"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46198-0"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00296-18"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.238147"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-3260com"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01234-15"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.07.004"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.05.082"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.147"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/v9090249"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw668"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2006.00869.x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-008-0639-6"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpim.51"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/4441022a"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.88"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathophys.2014.11.001"},{"key":"e_1_2_10_37_1","first-page":"1951","article-title":"Establishment of a novel mouse model of ulcerative colitis with concomitant cytomegalovirus infection: in vivo identification of cytomegalovirus persistent infected cells","volume":"19","author":"Matsumura K.","year":"2013","journal-title":"Inflamm. Bowel. Dis."},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.090471"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-019-01122-x"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-018-3792-z"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i12.3750"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-018-0041-4"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21072438"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652408784221306"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006202"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0000000000000481"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20042"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0174695"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(93)90329-6"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00545-11"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(00)00202-0"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1203516"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.061994-0"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.01.070"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01906-15"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33721"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.131"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2836"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-9-123"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgr256"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-19-0312"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.202249940","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/eji.202249940","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.202249940","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T06:45:52Z","timestamp":1692427552000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.202249940"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,4]]},"references-count":60,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1002\/eji.202249940"],"URL":"https:\/\/doi.org\/10.1002\/eji.202249940","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2022.03.17.484735","asserted-by":"object"}]},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,4]]},"assertion":[{"value":"2022-04-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2249940"}}