{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:22:38Z","timestamp":1790515358571,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T00:00:00Z","timestamp":1670457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Flagellin\u2010induced NAIP\/NLRC4 inflammasome activation and pyroptosis are critical events restricting\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    infection. However, the cellular and molecular dynamics of the\n                    <jats:italic>in vivo<\/jats:italic>\n                    responses against this bacterium are still unclear. We have found temporal coordination of two independent innate immunity pathways in controlling\n                    <jats:italic>Legionella<\/jats:italic>\n                    infection, the inflammasome activation and the CCR2\u2010mediated Mo\u2010DC recruitment. Inflammasome activation was an important player at the early stage of infection by lowering the numbers of bacteria for an efficient bacterial clearance conferred by the Mo\u2010DC at the late stage of the infection. Mo\u2010DC emergence highly depended on CCR2\u2010signaling and dispensed inflammasome activation and pyroptosis. Also, Mo\u2010DC compartment did not rely on the inflammasome machinery to deliver proper immune responses and was the most abundant cytokine\u2010producing among the monocyte\u2010derived cells in the infected lung. Importantly, when the CCR2\u2010 and NLRC4\u2010dependent axes of response were simultaneously ablated, we observed an aggravated bacterial burden in the lung of infected mice. Taken together, we showed that inflammasome activation and CCR2\u2010mediated immune response interplay in distinct pathways to restrict pulmonary bacterial infection. These findings extend our understanding of the in vivo integration and cooperation of different innate immunity arms in controlling infectious agents.\n                  <\/jats:p>","DOI":"10.1002\/eji.202249985","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T18:55:47Z","timestamp":1669402547000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Inflammasome activation and CCR2\u2010mediated monocyte\u2010derived dendritic cell recruitment restrict\n                    <i>Legionella pneumophila<\/i>\n                    infection"],"prefix":"10.1002","volume":"53","author":[{"given":"Marco A.","family":"Ataide","sequence":"first","affiliation":[{"name":"Department of Cell Biology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graziele Z.","family":"Manin","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel S.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhanoica O.","family":"Guerra","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7856-7512","authenticated-orcid":false,"given":"Dario S.","family":"Zamboni","sequence":"additional","affiliation":[{"name":"Department of Cell 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