{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:09:38Z","timestamp":1772118578720,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T00:00:00Z","timestamp":1670457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BE2813\/1\u20102"],"award-info":[{"award-number":["BE2813\/1\u20102"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The lymphocyte\u2010specific adapter protein SLy1 has previously been identified as indispensable for thymocyte development and T\u2010cell proliferation and, recently, as a cause of X\u2010linked combined immunodeficiency in humans that recapitulates many of the abnormalities reported in SLy1\n                    <jats:sup>KO<\/jats:sup>\n                    and SLy1\n                    <jats:sup>d\/d<\/jats:sup>\n                    mice. As SLy1\n                    <jats:sup>KO<\/jats:sup>\n                    NK cells show increased levels of p53, we focused our research on the interdependency of SLy1 and p53 for thymocyte development. Using RT\u2010PCR and immunoblot analysis, we observed increased levels of p53 as well as DNA damage response proteins in SLy1\n                    <jats:sup>KO<\/jats:sup>\n                    thymocytes. To test for rescue from SLy1\u2010induced deficiencies in thymocyte development like reduced thymocyte numbers and reduced DN to DP progression, we generated a mouse model with T cell\u2010specific p53\u2010deficiency on an SLy1\n                    <jats:sup>KO<\/jats:sup>\n                    background and analyzed lymphocyte populations in these mice and respective controls. Astonishingly, SLy1\n                    <jats:sup>KO<\/jats:sup>\n                    \u2010typical deficiencies were retained, showing that SLy1 is mechanistically independent of p53. Studies of apoptosis and proliferation in SLy1KO thymocytes revealed decreased proliferation in the DN3 subpopulation as a possible reason for the decreased thymocyte number. In mice with p53\u2010deficient T cells, we observed tumor formation leading to reduced survival, preferentially in SLy1\n                    <jats:sup>WT<\/jats:sup>\n                    mice. Thus, we suggest that a SLy1\u2010deficiency reduces proliferation, resulting in less hematologic tumors initiated by the p53\u2010deficiency.\n                  <\/jats:p>","DOI":"10.1002\/eji.202250017","type":"journal-article","created":{"date-parts":[[2022,11,19]],"date-time":"2022-11-19T09:48:40Z","timestamp":1668851320000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Knockout of SLy1 decreases double\u2010negative thymocyte proliferation and protects mice from p53\u2010induced tumor formation"],"prefix":"10.1002","volume":"53","author":[{"given":"Lena\u2010Christin","family":"Gruber","sequence":"first","affiliation":[{"name":"Department of Pharmacology Experimental Therapy and Toxicology Institute of Experimental and Clinical Pharmacology and Pharmacogenomik and ICePhA University of Tuebingen  Tuebingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Experimental Therapy and Toxicology Institute of Experimental and Clinical Pharmacology and Pharmacogenomik and ICePhA University of Tuebingen  Tuebingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christin","family":"Nothnagel","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Experimental Therapy and Toxicology Institute of Experimental and Clinical Pharmacology and Pharmacogenomik and ICePhA University of Tuebingen  Tuebingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6049-0257","authenticated-orcid":false,"given":"Sandra","family":"Beer\u2010Hammer","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Experimental Therapy and Toxicology Institute of Experimental and Clinical Pharmacology and Pharmacogenomik and ICePhA University of Tuebingen  Tuebingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,12,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.21.9646-9660.2005"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202002495R"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-012-0081-z"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560060128"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020008629"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-10-38"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201545609"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2016.1238543"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/356215a0"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08467"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.3.289"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.3.278"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/3882"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-380888-2.00003-0"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.035105"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.13.4129-4139.2001"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.2.152"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80416-X"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.04.012"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.10.036"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.2940140105"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.8.994"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.15.6861"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201970107"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(04)75025-6"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.10003"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1309\/K2FY1TED8GEGFLNG"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2016.10.007"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1600827"}],"container-title":["European 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