{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:23:13Z","timestamp":1787509393013,"version":"build-2736575974"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T00:00:00Z","timestamp":1668729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010345","name":"B. Braun-Stiftung","doi-asserted-by":"publisher","award":["BBST\u2010D\u201016\u201000071"],"award-info":[{"award-number":["BBST\u2010D\u201016\u201000071"]}],"id":[{"id":"10.13039\/100010345","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Germany`s Excellence Strategy \u2013 EXC 2155 \u2018RESI"],"award-info":[{"award-number":["Germany`s Excellence Strategy \u2013 EXC 2155 \u2018RESI"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Nowadays laparoscopic interventions enable the collection of resident macrophage populations out of the human cavities. We employed this technique to isolate pleural monocytes\/macrophages from healthy young adults who underwent a correction of\n                    <jats:italic>pectus excavatum<\/jats:italic>\n                    . High quality CD14\n                    <jats:sup>+<\/jats:sup>\n                    monocytes\/macrophages (plMo\/M\u03c6) were used for RNA\u2010sequencing (RNA\u2010seq) in comparison with human monocyte\u2010derived macrophages (MDM) natural (MDM\u20100) or IL\u20104\u2010polarized (MDM\u2010IL4). Transcriptome analysis revealed 7166 and 7076 differentially expressed genes (DEGs) in plMo\/M\u03c6 relative to natural MDM\u20100 and polarized MDM\u2010IL4, respectively. The gene set enrichment analysis, which was used to compare RNA\u2010seq data from plMo\/M\u03c6 with single\u2010cell (scRNA\u2010seq) data online from human bronchial lavage macrophages, showed that plMo\/M\u03c6s are characterized by a high expression of genes belonging to the metallothionein (MT) family, and that the expression of these genes is significantly higher in plMo\/M\u03c6 than in MDM\u20100 or MDM\u2010IL4. Our results provide additional insights on high MTs\u2010expressing macrophage subsets, which seem to be present not only in bronchial lavage of healthy adults or in pleural exudates of lung cancer patients but also in pleural fluid of healthy young adults. Macrophage subsets expressing high MTs may have specific roles in lung defense, repair, and homeostasis, and require further investigations.\n                  <\/jats:p>","DOI":"10.1002\/eji.202250019","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T05:44:23Z","timestamp":1667367863000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Pleural CD14\n                    <sup>+<\/sup>\n                    monocytes\/macrophages of healthy adolescents show a high expression of metallothionein family genes"],"prefix":"10.1002","volume":"53","author":[{"given":"Nagoud","family":"Schukfeh","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery Hannover Medical School  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Hannover Medical School  Hannover Germany"},{"name":"Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) Member of the German Center for Lung Research (DZL)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"DeLuca","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Hannover Medical School  Hannover Germany"},{"name":"Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) Member of the German Center for Lung Research (DZL)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srinu","family":"Tumpara","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Hannover Medical School  Hannover Germany"},{"name":"Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) Member of the German Center for Lung Research (DZL)  Hannover 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