{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T01:04:02Z","timestamp":1775610242526,"version":"3.50.1"},"reference-count":86,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T00:00:00Z","timestamp":1672704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Several subsets of mononuclear phagocytes and DCs (MDC) populate the small intestine (SI), and these cells reportedly exert specialized functions in anti\u2010microbial immunity and tolerance. Given the specialized phenotype of these cells, differing from other MDC family members, including their putative circulating blood precursors, local intestinal factors play key instructive roles in their differentiation. We designed an SI cell culture model composed of three intestinal epithelial cell (IEC) types, including absorptive enterocytes (E cells), antigen delivering microfold (M) cells, and mucus\u2010producing goblet (G) cells plus T lymphocytes and soluble B cell\u2010derived factors. This model was used to study the differentiation fate of CD34\n                    <jats:sup>+<\/jats:sup>\n                    hematopoietic progenitor cell\u2010derived monocyte\/DC precursors. Progeny cells can be analyzed after a 3\u2010week co\u2010culture period, mimicking the physiologic turn\u2010over time of intestinal MDC. A dominant monocyte differentiation pathway was suppressed, in favor of partial differentiation along DC and macrophage pathways, with low percentages of cells acquired DC or macrophage markers. Moreover, E and G cells play opposing roles in CX3CR1\n                    <jats:sup>+<\/jats:sup>\n                    vs CD103\n                    <jats:sup>dim<\/jats:sup>\n                    cell differentiation, indicating that both together might counter\u2010balance M\/DC differentiation. Thus, SI epithelial cells suppress M\/DC differentiation, supporting a key role for exogenous factors in M\/DC differentiation.\n                  <\/jats:p>","DOI":"10.1002\/eji.202250131","type":"journal-article","created":{"date-parts":[[2022,12,17]],"date-time":"2022-12-17T00:08:21Z","timestamp":1671235701000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A novel 3D cell culture model to study the human small intestinal immune landscape"],"prefix":"10.1002","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3706-9514","authenticated-orcid":false,"given":"Christa","family":"Schimpel","sequence":"first","affiliation":[{"name":"Medical University of Graz, Otto Loewi Research Center for Vascular Biology, Immunology and Inflammation, Division of Immunology and Pathophysiology  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Passegger","sequence":"additional","affiliation":[{"name":"Medical University of Graz, Otto Loewi Research Center for Vascular Biology, Immunology and Inflammation, Division of Immunology and Pathophysiology  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Egger","sequence":"additional","affiliation":[{"name":"Medical University of Graz, Otto Loewi Research Center for Vascular Biology, Immunology and Inflammation, Division of Immunology and Pathophysiology  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Tam\u2010Amersdorfer","sequence":"additional","affiliation":[{"name":"Medical University of Graz, Otto Loewi Research Center for Vascular Biology, Immunology and Inflammation, Division of Immunology and Pathophysiology  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8070-4977","authenticated-orcid":false,"given":"Herbert","family":"Strobl","sequence":"additional","affiliation":[{"name":"Medical University of Graz, Otto Loewi Research Center for Vascular Biology, Immunology and Inflammation, Division of Immunology and Pathophysiology  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2017.04.004"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-013-0929-4"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/17474124.2017.1343143"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2008.175166"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1192"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.70.2.241"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1102084"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/86373"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2014.03.017"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx300295n"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6017(200001)89:1<63::AID-JPS7>3.0.CO;2-6"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20171497"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-04-348946"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2013.30"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.6b01165"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp400507g"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2012.3"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2013.10.003"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2012.10.003"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2016.07.051"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2005.04.015"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/adt.2014.573"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/teb.2007.0150"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2015.64"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2768"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00658-6"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600085"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.08.013"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0176460"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.06.075"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep29783"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2017.08.011"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-020-02750-1"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp1000795"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-021-00279-y"},{"issue":"1","key":"e_1_2_9_37_1","first-page":"1","article-title":"Self\u2010organized intestinal epithelial monolayers in crypt and villus\u2010like domains show effective barrier function","volume":"9","author":"Altay G.","year":"2019","journal-title":"Sci. Rep."},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2006.12.006"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.106.3.771"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3863"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(00)00128-9"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(89)80072-1"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-005-0085-6"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1515\/entl-2015-0006"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3762\/bjnano.6.151"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)01266-1"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvv121"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/v10020065"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02035"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01761"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.022418"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.52.10.1522"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1556\/EuJMI.1.2011.1.5"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00174-2"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00410"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.4049\/immunohorizons.1900038"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2014.03.012"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.05.068"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2012.79"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2007.10.009"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20101967"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2007.02687.x"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00254"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2014.51"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu9010068"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2012.125"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21144825"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11148-2"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI59150"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201141714"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.4.2465"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.4110\/in.2018.18.e5"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.01.009"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9102162"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2011.06.003"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2011.00025"},{"key":"e_1_2_9_77_1","unstructured":"Tolerogenic dendritic cells.Proceedings of the Seminars in immunopathology2017. Springer."},{"key":"e_1_2_9_78_1","first-page":"1","article-title":"Dendritic cell functions in the inductive and effector sites of intestinal immunity","author":"Luciani C.","year":"2021","journal-title":"Mucosal Immunol"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201948497"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/1958650"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3408"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25115-3"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10863"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.6.3745"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2009.13"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051100"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2018020123"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.202250131","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/eji.202250131","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.202250131","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T17:48:26Z","timestamp":1692640106000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.202250131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,3]]},"references-count":86,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1002\/eji.202250131"],"URL":"https:\/\/doi.org\/10.1002\/eji.202250131","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/EJI.202250131\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250131\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250131\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250131\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250131\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250131\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250131\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250131\/v3\/decision1","asserted-by":"object"}]},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,3]]},"assertion":[{"value":"2022-08-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2250131"}}