{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T19:17:12Z","timestamp":1789067832678,"version":"build-2803163510"},"reference-count":57,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T00:00:00Z","timestamp":1687910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Enterotoxigenic\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (ETEC) is an important cause of children's and travelers\u2019 diarrhea, with no licensed vaccine. This study aimed to explore the role of cellular immunity in protection against human ETEC infection. Nine volunteers were experimentally infected with ETEC, of which six developed diarrhea. Lymphocytes were collected from peripheral blood buffy coats, before and 3, 5, 6, 7, 10, and 28 days after dose ingestion, and 34 phenotypic and functional markers were examined by mass cytometry. Thirty\u2010three cell populations, derived by manually merging 139 cell clusters from the X\u2010shift unsupervised clustering algorithm, were analyzed. Initially, the diarrhea group responded with increased CD56dim CD16\n                    <jats:sup>+<\/jats:sup>\n                    natural killer cells, dendritic cells tended to rise, and mucosal\u2010associated invariant T cells decreased. On day 5\u20107, an increase in plasmablasts was paralleled by a consistent rise in CD4\n                    <jats:sup>+<\/jats:sup>\n                    Th17\u2010like effector memory and regulatory cell subsets. CD4\n                    <jats:sup>+<\/jats:sup>\n                    Th17\u2010like central memory cells peaked on day 10. All Th17\u2010like cell populations showed increased expression of activation, gut\u2010homing, and proliferation markers. Interestingly, in the nondiarrhea group, these same CD4\n                    <jats:sup>+<\/jats:sup>\n                    Th17\u2010like cell populations expanded earlier, normalizing around day 7. Earlier development of these CD4\n                    <jats:sup>+<\/jats:sup>\n                    Th17\u2010like cell populations in the nondiarrhea group may suggest a recall response and a potential role in controlling ETEC infections.\n                  <\/jats:p>","DOI":"10.1002\/eji.202250254","type":"journal-article","created":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T06:34:25Z","timestamp":1682577265000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Dynamics of circulating lymphocytes responding to human experimental enterotoxigenic\n                    <i>Escherichia coli<\/i>\n                    infection"],"prefix":"10.1002","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5942-2422","authenticated-orcid":false,"given":"Sehee","family":"Rim","sequence":"first","affiliation":[{"name":"Department of Clinical Science Faculty of Medicine University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunniva T.","family":"Sakkestad","sequence":"additional","affiliation":[{"name":"Department of Clinical Science Faculty of Medicine University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Clinical Science Faculty of Medicine University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stein\u2010Erik","family":"Gullaksen","sequence":"additional","affiliation":[{"name":"Department of Clinical Science Centre of Cancer Biomarkers (CCBIO) University of Bergen Bergen Norway"},{"name":"Hematology Section Department of Internal Medicine Helse Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f8rn","family":"Skavland","sequence":"additional","affiliation":[{"name":"Department of Clinical Science Faculty of Medicine University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudhir K.","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Division of Cancer Medicine Department of Cancer Immunology Oslo University Hospital Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Steinsland","sequence":"additional","affiliation":[{"name":"Department of Global Public Health and Primary Care Faculty of Medicine Centre for Intervention Science in Maternal and Child Health (CISMAC) Centre for International Health University of Bergen Bergen Norway"},{"name":"Department of Biomedicine Faculty of Medicine University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1466-2326","authenticated-orcid":false,"given":"Kurt","family":"Hanevik","sequence":"additional","affiliation":[{"name":"Department of Clinical Science Faculty of Medicine University of Bergen Bergen Norway"},{"name":"Department of Medicine Norwegian National Advisory Unit on Tropical Infectious Diseases Haukeland University Hospital Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30475-4"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.11.049"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.10.002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2019.03.040"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.101"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12673"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2010.11.002"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01014"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051718"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2017.70"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.3.874-879.1994"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007823"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005291"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-20740-3"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M207577200"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041257"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070663"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201242741"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2010.07.002"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010018\u20130133\u2010z"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004914"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens12020283"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/44385"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.9.1407"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20080397"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-015-9457-4"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.06.012"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00302"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106443200"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-02886-8"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42861-z"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.204.Supp.148.25"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1890"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00398"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.1.221"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00341"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05088-11"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.08.058"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00628.x"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00685"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-015-0264-2"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1181"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.3.482-488.2001"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838908"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens8020084"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.23.3.729-736.1979"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21994"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9650-6_4"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2020.112940"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02432-09"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05194-11"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.23751"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2019.112673"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3863"},{"key":"e_1_2_10_56_1","doi-asserted-by":"crossref","unstructured":"Seabold S.andPerktoldJ. Statsmodels: econometric and statistical modeling with python.Proceedings of the 9th Python in Science Conference 2010.","DOI":"10.25080\/Majora-92bf1922-011"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.21105\/joss.03021"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.4028\/www.scientific.net\/AMM.472.466"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.202250254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,11]],"date-time":"2023-08-11T05:37:38Z","timestamp":1691732258000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.202250254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,28]]},"references-count":57,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1002\/eji.202250254"],"URL":"https:\/\/doi.org\/10.1002\/eji.202250254","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/EJI.202250254\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250254\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250254\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250254\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250254\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250254\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250254\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250254\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202250254\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,28]]},"assertion":[{"value":"2022-11-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2250254"}}