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However, the study of LNSCs in human diseases is complicated by a dependence on viable lymphoid tissues, which are most often excised prior to establishment of a specific diagnosis. Here, we demonstrate that cryopreservation can be used to bank lymphoid tissue for the study of LNSCs in human disease. Using human tonsils and lymph nodes (LN), lymphoid tissue fragments were cryopreserved for subsequent enzymatic digestion and recovery of viable nonhematopoietic cells. Flow cytometry and single\u2010cell transcriptomics identified comparable proportions of LN stromal cell types in fresh and cryopreserved tissue. Moreover, cryopreservation had little effect on transcriptional profiles, which showed significant overlap between tonsils and LN. The presence and spatial distribution of transcriptionally defined cell types were confirmed by in situ analyses. Our broadly applicable approach promises to greatly enable research into the roles of LNSCs in human disease.<\/jats:p>","DOI":"10.1002\/eji.202250362","type":"journal-article","created":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T06:56:21Z","timestamp":1687848981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A novel cryopreservation and biobanking strategy to study lymphoid tissue stromal cells in human disease"],"prefix":"10.1002","volume":"53","author":[{"given":"Joshua D.","family":"Brandstadter","sequence":"first","affiliation":[{"name":"Division of Hematology\/Oncology Perelman School of Medicine University of Pennsylvania  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelina","family":"De Martin","sequence":"additional","affiliation":[{"name":"Institute of Immunobiology Kantonsspital St. Gallen  St. Gallen 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