{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:08:47Z","timestamp":1792188527878,"version":"4.4.0"},"reference-count":71,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100017957","name":"Health Commission of Guizhou Province","doi-asserted-by":"publisher","award":["gzwkj2023\u2010568"],"award-info":[{"award-number":["gzwkj2023\u2010568"]}],"id":[{"id":"10.13039\/100017957","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31570910"],"award-info":[{"award-number":["31570910"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770993"],"award-info":[{"award-number":["31770993"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170929"],"award-info":[{"award-number":["32170929"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32260189"],"award-info":[{"award-number":["32260189"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32300772"],"award-info":[{"award-number":["32300772"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32370979"],"award-info":[{"award-number":["32370979"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82301980"],"award-info":[{"award-number":["82301980"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["91742104"],"award-info":[{"award-number":["91742104"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004001","name":"Guizhou Provincial Science and Technology Department","doi-asserted-by":"publisher","award":["ZK2024\u2010240"],"award-info":[{"award-number":["ZK2024\u2010240"]}],"id":[{"id":"10.13039\/501100004001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["20ZR1443800"],"award-info":[{"award-number":["20ZR1443800"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["21ZR1405400"],"award-info":[{"award-number":["21ZR1405400"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["22ZR1448800"],"award-info":[{"award-number":["22ZR1448800"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Alveolar macrophages (AMs) are sentinels in the airways, where they sense and respond to invading microbes and other stimuli. Unlike macrophages in other locations, AMs can remain responsive to Gram\u2010negative lipopolysaccharides (LPS) after they have responded to LPS in vivo (they do not develop \u201cendotoxin tolerance\u201d), suggesting that the alveolar microenvironment may influence their responses. Although alveolar epithelial cells (AECs) normally limit AMs\u2019 innate responses, preventing inflammation induced by harmless antigens in the lung, how AECs influence the innate responses of AMs to infectious agents has been uncertain. Here we report that (1) after exposure to aspirated (intranasal instillation) LPS, AMs increase their responses to TLR agonists and elevate their phagocytic and bactericidal activities in mice; (2) Aspirated LPS pre\u2010exposure increases host resistance to pulmonary infection caused by Gram\u2010negative bacteria and the protection effect lasts for at least 35 days; (3) LPS stimulation of AECs both increases AMs\u2019 innate immune responses and prevents AMs from developing tolerance in vitro; (4) Upon LPS stimulation, AMs secreted TNF\u2010\u03b1 induces AECs to release GM\u2010CSF, which potentiates AMs\u2019 response. These experiments have revealed a previously unappreciated role that AECs may play in boosting the innate responses of AMs and promoting resistance to pulmonary infections.<\/jats:p>","DOI":"10.1002\/eji.202350378","type":"journal-article","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T05:52:35Z","timestamp":1730785955000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Alveolar epithelial cells shape lipopolysaccharide\u2010induced inflammatory responses and reprogramming of alveolar macrophages"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7698-3857","authenticated-orcid":false,"given":"Wei","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences Department of Trauma\u2010Emergency &amp; Critical Care Medicine Shanghai Fifth People's Hospital Shanghai Institute of Infectious Disease and Biosecurity Fudan University  Shanghai China"},{"name":"Department of Rheumatology and Immunology the Affiliated Hospital of Guizhou Medical University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeying","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences Department of Trauma\u2010Emergency &amp; Critical Care Medicine Shanghai Fifth People's Hospital Shanghai Institute of Infectious Disease and Biosecurity Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Yun","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences Department of Trauma\u2010Emergency &amp; Critical Care Medicine Shanghai Fifth People's Hospital Shanghai Institute of Infectious Disease and Biosecurity Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benkun","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences Department of Trauma\u2010Emergency &amp; Critical Care Medicine Shanghai Fifth People's Hospital Shanghai Institute of Infectious Disease and Biosecurity Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimeng","family":"Lu","sequence":"additional","affiliation":[{"name":"Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihui","family":"Tang","sequence":"additional","affiliation":[{"name":"Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiying","family":"Mao","sequence":"additional","affiliation":[{"name":"Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Han","sequence":"additional","affiliation":[{"name":"Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyun","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response National Medical Center for Infectious Diseases, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiashun","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology the Affiliated Hospital of Guizhou Medical University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwei","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences Department of Trauma\u2010Emergency &amp; Critical Care Medicine Shanghai Fifth People's Hospital Shanghai Institute of Infectious Disease and Biosecurity Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianguo","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Trauma\u2010Emergency &amp; Critical Care Medicine, Shanghai Fifth People's Hospital Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences Department of Trauma\u2010Emergency &amp; Critical Care Medicine Shanghai Fifth People's Hospital Shanghai Institute of Infectious Disease and Biosecurity Fudan University  Shanghai China"},{"name":"MOE Innovative Center for New Drug Development of Immune Inflammatory Diseases Fudan University  Shanghai China"},{"name":"Shanghai Sci\u2010Tech Inno Center for Infection &amp; Immunity  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3052"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-021014-071937"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-023-00848-y"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2005-0246OC"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5212"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004053"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.5.2602"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12902"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-016-0569-x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3600"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3325"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3306"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc13110"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2009.07.009"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1086\/382657"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e31825b8d57"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/09680519060120030301"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2008.06.009"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2008-0362OC"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01541347"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(78)90574-0"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00758-X"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20062611"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06002-x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI29919"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1309"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011556"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201011-1891OC"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006436"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.158.6.2862"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003339"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2009.09.004"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3005"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.1.463"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200711-1661OC"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1637"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01413"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.06.006"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.09.034"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-022-00633-5"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf1098"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-0285-6"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3106"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.69951"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3857"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-022-00528-5"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.09.042"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3581"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021827"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3045"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20210745"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.10.026"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8171324"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200705200"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-031210-101303"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.05.047"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02738"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1383206"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI35412"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1765"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070891"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200607-1038OC"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI40817"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.12.002"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000172277.41033.F0"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.11.7574"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20221388"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2017.71"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119798"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-7364-3-4-361"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.23.11029"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.202350378","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,23]],"date-time":"2025-02-23T08:00:55Z","timestamp":1740297655000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.202350378"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,5]]},"references-count":71,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/eji.202350378"],"URL":"https:\/\/doi.org\/10.1002\/eji.202350378","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/EJI.202350378\/v4\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202350378\/v4\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202350378\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202350378\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202350378\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202350378\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202350378\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202350378\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202350378\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202350378\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.202350378\/v1\/review2","asserted-by":"object"}]},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,5]]},"assertion":[{"value":"2024-02-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2350378"}}