{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T17:23:16Z","timestamp":1778260996811,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T00:00:00Z","timestamp":1689120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201020\u2010COV8\u20100004\u201001"],"award-info":[{"award-number":["ANR\u201020\u2010COV8\u20100004\u201001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201020\u2010CE15\u20100014\u201001"],"award-info":[{"award-number":["ANR\u201020\u2010CE15\u20100014\u201001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Toll\u2010like receptor 7 (TLR7) triggers antiviral immune responses through its capacity to recognize single\u2010stranded RNA.\n                    <jats:italic>TLR7<\/jats:italic>\n                    loss\u2010of\u2010function mutants are associated with life\u2010threatening pneumonia in severe COVID\u201019 patients. Whereas TLR7\u2010driven innate induction of type I IFN appears central to control SARS\u2010CoV2 virus spreading during the first days of infection, the impact of\n                    <jats:italic>TLR7<\/jats:italic>\n                    \u2010deficiency on adaptive B\u2010cell immunity is less clear. In the present study, we examined the role of TLR7 in the adaptive B cells response to various pathogen\u2010like antigens (PLAs). We used inactivated SARS\u2010CoV2 and a PLA\u2010based COVID\u201019 vaccine candidate designed to mimic SARS\u2010CoV2 with encapsulated bacterial ssRNA as TLR7 ligands and conjugated with the RBD of the SARS\u2010CoV2 Spike protein. Upon repeated immunization with inactivated SARS\u2010CoV2 or PLA COVID\u201019 vaccine, we show that\n                    <jats:italic>Tlr7<\/jats:italic>\n                    \u2010deficiency abolished the germinal center (GC)\u2010dependent production of RBD\u2010specific class\u2010switched IgG2b and IgG2c, and neutralizing antibodies to SARS\u2010CoV2. We also provide evidence for a non\u2010redundant role for B\u2010cell\u2010intrinsic TLR7 in the promotion of RBD\u2010specific IgG2b\/IgG2c and memory B cells. Together, these data demonstrate that the GC reaction and class\u2010switch recombination to the Myd88\u2010dependent IgG2b\/IgG2c in response to SARS\u2010CoV2 or PLAs is strictly dependent on cell\u2010intrinsic activation of TLR7 in B cells.\n                  <\/jats:p>","DOI":"10.1002\/eji.202350437","type":"journal-article","created":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T01:57:06Z","timestamp":1689213426000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["B cell\u2010intrinsic TLR7 signaling is required for neutralizing antibody responses to SARS\u2010CoV\u20102 and pathogen\u2010like COVID\u201019 vaccines"],"prefix":"10.1002","volume":"53","author":[{"given":"Charles\u2010Henry","family":"Miquel","sequence":"first","affiliation":[{"name":"Institut Toulousain des Maladies Infectieuses et Inflammatoires (INFINITY) Universit\u00e9 de Toulouse  INSERM, CNRS, UPS Toulouse 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