{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:43:25Z","timestamp":1780101805867,"version":"3.54.0"},"reference-count":69,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T00:00:00Z","timestamp":1707868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The Wiskott\u2013Aldrich syndrome protein (WASp) regulates actin cytoskeletal dynamics and function of hematopoietic cells. Mutations in the\n                    <jats:italic>WAS<\/jats:italic>\n                    gene lead to two different syndromes; Wiskott\u2013Aldrich syndrome (WAS) caused by loss\u2010of\u2010function mutations, and X\u2010linked neutropenia (XLN) caused by gain\u2010of\u2010function mutations. We previously showed that WASp\u2010deficient mice have a decreased number of regulatory T (Treg) cells in the thymus and the periphery. We here evaluated the impact of WASp mutations on Treg cells in the thymus of WAS and XLN mouse models. Using in vitro Treg differentiation assays, WAS CD4 single\u2010positive thymocytes have decreased differentiation to Treg cells, despite normal early signaling upon IL\u20102 and TGF\u2010\u03b2 stimulation. They failed to proliferate and express CD25 at high levels, leading to poor survival and a lower number of Foxp3\n                    <jats:sup>+<\/jats:sup>\n                    Treg cells. Conversely, XLN CD4 single\u2010positive thymocytes efficiently differentiate into Foxp3\n                    <jats:sup>+<\/jats:sup>\n                    Treg cells following a high proliferative response to IL\u20102 and TGF\u2010\u03b2, associated with high CD25 expression when compared with WT cells. Altogether, these results show that specific mutations of WASp affect Treg cell development differently, demonstrating a critical role of WASp activity in supporting Treg cell development and expansion.\n                  <\/jats:p>","DOI":"10.1002\/eji.202350450","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T00:47:13Z","timestamp":1707958033000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Controlled WASp activity regulates the proliferative response for Treg cell differentiation in the thymus"],"prefix":"10.1002","volume":"54","author":[{"given":"Larissa","family":"Vasconcelos\u2010Fontes","sequence":"first","affiliation":[{"name":"Department of Microbiology Tumor and Cell Biology Karolinska Institutet  Stockholm Sweden"},{"name":"Laboratory on Thymus Research Oswaldo Cruz Institute Fiocruz  Rio de Janeiro Brazil"},{"name":"National Institute of Science and Technology 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