{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T15:35:17Z","timestamp":1767713717797,"version":"3.41.2"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T00:00:00Z","timestamp":1715299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Loss of perfusion in the burn wound might cause wound deepening and impaired healing. We previously showed persistent microvascular thrombosis coinciding with intraluminal neutrophils extracellular traps in human burned skin. This study investigates the presence of intraluminal citrullinated histone 3 (H3cit) from different cellular origins (neutrophils, monocytes, and lymphocytes) in relation to microvascular thrombosis of burn wounds. Eschar was obtained from burn patients (<jats:italic>n<\/jats:italic> = 18) 6\u201340 days postburn with a mean total burned body surface area of 23%. Microvascular presence of tissue factor (TF), factor XII (FXII) and thrombi was assessed by immunohistochemistry. Intramicrovascular cell death was analyzed via immunofluorescent microscopy, combining antibodies for neutrophils (MPO), monocytes (CD14), and lymphocytes (CD45) with endothelial cell markers CD31 and H3cit. Significantly increased microvascular expression of TF, FXII, and thrombi (CD31<jats:sup>+<\/jats:sup>) was found in all eschar samples compared with control uninjured skin. Release of H3cit from different cellular origins was observed in the lumen of the dermal microvasculature in the eschar tissue 7\u201340 days postburn, with release from neutrophilic origin being 2.7 times more abundant. Intraluminal presence of extracellular H3cit colocalizing with either MPO, CD14, or CD45 is correlated to increased microvascular thrombosis in eschar of burn patients.<\/jats:p>","DOI":"10.1002\/eji.202350792","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T12:35:10Z","timestamp":1715344510000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Intraluminal release of citrullinated histone 3 from various cellular origins coincides with microvascular thrombosis in burn wounds"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7858-2771","authenticated-orcid":false,"given":"Britt","family":"van der Leeden","sequence":"first","affiliation":[{"name":"Pathology Amsterdam UMC location Vrije Universiteit Amsterdam  Amsterdam the Netherlands"},{"name":"Inflammatory Diseases Amsterdam Institute for Infection and Immunity  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Ibrahim","family":"Korkmaz","sequence":"additional","affiliation":[{"name":"Association of Dutch Burn Centers  Beverwijk the Netherlands"},{"name":"Molecular cell biology and Immunology Amsterdam UMC location Vrije Universiteit Amsterdam  Amsterdam the Netherlands"},{"name":"Burn Center and Department of Plastic, Reconstructive and Hand Surgery Red Cross Hospital  Beverwijk the Netherlands"},{"name":"Department of Plastic, Reconstructive and Hand Surgery Amsterdam UMC location Vrije Universtiteit Amsterdam  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"Vlig","sequence":"additional","affiliation":[{"name":"Association of Dutch Burn Centers  Beverwijk the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingeborg S.E.","family":"Waas","sequence":"additional","affiliation":[{"name":"Pathology Amsterdam UMC location Vrije Universiteit Amsterdam  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bouke K.H.L.","family":"Boekema","sequence":"additional","affiliation":[{"name":"Association of Dutch Burn Centers  Beverwijk the Netherlands"},{"name":"Burn Center and Department of Plastic, Reconstructive and Hand Surgery Red Cross Hospital  Beverwijk the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chopie","family":"Hassan","sequence":"additional","affiliation":[{"name":"Pharming Technologies B.V.  Leiden the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul P.M.","family":"van Zuijlen","sequence":"additional","affiliation":[{"name":"Burn Center and Department of Plastic, Reconstructive and Hand Surgery Red Cross Hospital  Beverwijk the Netherlands"},{"name":"Department of Plastic, Reconstructive and Hand Surgery Amsterdam UMC location Vrije Universtiteit Amsterdam  Amsterdam the Netherlands"},{"name":"Amsterdam Movement Sciences Institute Amsterdam UMC  Amsterdam the Netherlands"},{"name":"Amsterdam UMC location University of Amsterdam, Pediatric Surgical Centre Emma Children's Hospital  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans W.M.","family":"Niessen","sequence":"additional","affiliation":[{"name":"Pathology Amsterdam UMC location Vrije Universiteit Amsterdam  Amsterdam the Netherlands"},{"name":"Department of Cardiac Surgery AUMC  Amsterdam the Netherlands"},{"name":"Amsterdam Cardiovascular 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