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Living primary tissue slices are ultrathin multicellular sections of the intestine and provide a unique opportunity to analyze tissue\u2010specific immune responses\n                    <jats:italic>ex vivo<\/jats:italic>\n                    . This study aimed to investigate whether supplementation of the gut flora with SFB promotes T helper 17 (Th17) cell responses in primary intestinal tissue slices\n                    <jats:italic>ex vivo<\/jats:italic>\n                    . Primary tissue slices were prepared from the small intestine of healthy\u00a0Taconic mice with SFB\u2010positive and SFB\u2010negative microbiomes and stimulated with anti\u2010CD3\/CD28 or Concanavalin A. SFB\u2010positive and \u2010negative mice exhibited distinct microbiome compositions and Th17 cell frequencies in the intestine and complex microbiota including SFB induced up to 15\u2010fold increase in Th17 cell\u2010associated mediators, serum amyloid A (SAA), and immunoglobulin A (IgA) responses\n                    <jats:italic>ex vivo<\/jats:italic>\n                    . This phenotype could be transmitted by co\u2010housing of mice. Our findings highlight that changes in the gut microbiome can be observed in primary intestinal tissue slices\n                    <jats:italic>ex vivo<\/jats:italic>\n                    . This makes the system very attractive for disease modeling and assessment of new therapies.\n                  <\/jats:p>","DOI":"10.1002\/eji.202350946","type":"journal-article","created":{"date-parts":[[2024,5,19]],"date-time":"2024-05-19T22:38:43Z","timestamp":1716158323000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Homeostatic T helper 17 cell responses triggered by complex microbiota are maintained in\n                    <i>ex vivo<\/i>\n                    intestinal tissue slices"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7044-1722","authenticated-orcid":false,"given":"Valerie","family":"Beneke","sequence":"first","affiliation":[{"name":"Division of Preclinical Pharmacology and Toxicology Fraunhofer Institute for Toxicology and Experimental Medicine  Hannover Germany"},{"name":"Member of the German Center for Lung Research (DZL) Biomedical Research in 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