{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T01:07:35Z","timestamp":1780103255116,"version":"3.54.0"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009553","name":"Agentura Pro Zdravotnick\u00fd V\u00fdzkum \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["23\u201007\u201000170"],"award-info":[{"award-number":["23\u201007\u201000170"]}],"id":[{"id":"10.13039\/501100009553","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Detailed knowledge of human B\u2010cell development is crucial for the proper interpretation of inborn errors of immunity and malignant diseases. It is of interest to understand the kinetics of protein expression changes during development, but also to properly interpret the major and possibly alternative developmental trajectories. We have investigated human samples from healthy individuals with the aim of describing all B\u2010cell developmental trajectories. We validated a 30\u2010parameter mass cytometry panel and demonstrated the utility of \u201c\n                    <jats:italic>vaevictis<\/jats:italic>\n                    \u201d visualization of B\u2010cell developmental stages. We used the trajectory inference tool \u201c\n                    <jats:italic>tviblindi<\/jats:italic>\n                    \u201d to exhaustively describe all trajectories leading to all developmental ends discovered in the data. Focusing on Natural Effector B cells, we demonstrated the dynamics of expression of nuclear factors (PAX\u20105, TdT, Ki\u201067, Bcl\u20102), cytokine and chemokine receptors (CD127, CXCR4, CXCR5) in relation to the canonical B\u2010cell developmental stage markers. We observed branching of the memory development, where follicular memory formation was marked by CD73 expression. Lastly, we performed an analysis of two example cases of abnormal B\u2010cell development caused by mutations in RAG\u20101 and Wiskott\u2013Aldrich syndrome gene in patients with primary immunodeficiency. In conclusion, we developed, validated, and presented a comprehensive set of tools for the investigation of B\u2010cell development in the bone marrow compartment.\n                  <\/jats:p>","DOI":"10.1002\/eji.202451004","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T11:42:41Z","timestamp":1725536561000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>Tviblindi<\/i>\n                    algorithm identifies branching developmental trajectories of human B\u2010cell development and describes abnormalities in RAG\u20101 and WAS patients"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5919-6306","authenticated-orcid":false,"given":"Marina","family":"Bakardjieva","sequence":"first","affiliation":[{"name":"CLIP Department of Paediatric Haematology and Oncology Second Faculty of Medicine Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ond\u0159ej","family":"Pel\u00e1k","sequence":"additional","affiliation":[{"name":"CLIP Department of Paediatric Haematology and Oncology Second Faculty of Medicine Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjolein","family":"Wentink","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Erasmus MC University Medical Center Rotterdam  Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hana","family":"Glier","sequence":"additional","affiliation":[{"name":"CLIP Department of Paediatric Haematology and Oncology Second Faculty of Medicine Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Nov\u00e1k","sequence":"additional","affiliation":[{"name":"CLIP Department of Paediatric Haematology and Oncology Second Faculty of Medicine Charles University  Prague Czech Republic"},{"name":"Department of Applied Mathematics Computer Science and Statistics Ghent University  Ghent Belgium"},{"name":"Data Mining and Modeling for Biomedicine Center for Inflammation Research VIB\u2010UGent  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitka","family":"Stan\u010d\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"CLIP Department of Paediatric Haematology and Oncology Second Faculty of Medicine Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8086-9790","authenticated-orcid":false,"given":"Daniela","family":"Ku\u017e\u00edlkov\u00e1","sequence":"additional","affiliation":[{"name":"CLIP Department of Paediatric Haematology and Oncology Second Faculty of Medicine Charles University  Prague Czech Republic"},{"name":"Department of Paediatric Haematology and Oncology University Hospital Motol  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ester","family":"Mejst\u0159\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"CLIP Department of Paediatric Haematology and Oncology Second Faculty of Medicine Charles University  Prague Czech Republic"},{"name":"Department of Paediatric Haematology and Oncology University Hospital Motol  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7526-2803","authenticated-orcid":false,"given":"Iga","family":"Janowska","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Freiburg University Medical Center University of Freiburg  Freiburg Germany"},{"name":"Center for Chronic Immunodeficiency University Medical Center Freiburg Faculty of Medicine University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5153-6089","authenticated-orcid":false,"given":"Marta","family":"Rizzi","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Freiburg University Medical Center University of Freiburg  Freiburg Germany"},{"name":"Center for Chronic Immunodeficiency University Medical Center Freiburg Faculty of Medicine University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1510-3104","authenticated-orcid":false,"given":"Mirjam","family":"van der Burg","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Laboratory for Pediatric Immmunology Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1896-9533","authenticated-orcid":false,"given":"Jan","family":"Stuchl\u00fd","sequence":"additional","affiliation":[{"name":"CLIP Department of Paediatric Haematology and Oncology Second Faculty of Medicine Charles University  Prague Czech Republic"},{"name":"Department of Paediatric Haematology and Oncology University Hospital Motol  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4475-2872","authenticated-orcid":false,"given":"Tom\u00e1\u0161","family":"Kalina","sequence":"additional","affiliation":[{"name":"CLIP Department of Paediatric Haematology and Oncology Second Faculty of Medicine Charles University  Prague Czech Republic"},{"name":"Department of Paediatric Haematology and Oncology University Hospital Motol  Prague Czech 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