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Here, we report the single\u2010cell RNA sequencing (scRNA\u2010seq) of 9267 LNSCs isolated from a human lymph node (LN). This study comprehensively defines the gene signatures of 10 fibroblast subtypes: CCL21\n                    <jats:sup>+<\/jats:sup>\n                    SC, CCL19\n                    <jats:sup>+<\/jats:sup>\n                    SC, CD34\n                    <jats:sup>+<\/jats:sup>\n                    CXCL14\n                    <jats:sup>+<\/jats:sup>\n                    SC, pericytes, DES\n                    <jats:sup>+<\/jats:sup>\n                    SC, LAMP5\n                    <jats:sup>+<\/jats:sup>\n                    SC, NR4A1\n                    <jats:sup>+<\/jats:sup>\n                    BCAM\n                    <jats:sup>+<\/jats:sup>\n                    SC, HLA\u2010DR\n                    <jats:sup>+<\/jats:sup>\n                    SC, SEPT4\n                    <jats:sup>+<\/jats:sup>\n                    SC and GLDN\n                    <jats:sup>+<\/jats:sup>\n                    SC. The existence of these subtypes was validated across 13 LN donors using 2 publicly available datasets and our dataset. To explore the heterogeneous stromal compartment within the complex LN tissue architecture, we integrated the scRNA\u2010seq profiles of the identified LNSC subsets with a publicly available human spatial transcriptomic LN dataset and predicted their location within the complex LN tissue architecture. Each LNSC subtype was spatially restricted to specific LN regions, indicating different LNSC\u2013lymphocyte interactions, which were further investigated using NicheNet. The positioning of distinct LNSC subtypes within different LN regions sets the stage for future research on the relationship between LNSC\u2010specific niches and immunomodulatory function during health and disease.\n                  <\/jats:p>","DOI":"10.1002\/eji.202451218","type":"journal-article","created":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T08:12:09Z","timestamp":1750147929000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification and Mapping of Human Lymph Node Stromal Cell Subsets by Combining Single\u2010Cell RNA Sequencing with Spatial Transcriptomics"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6605-6910","authenticated-orcid":false,"given":"Cristoforo","family":"Grasso","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology, Amsterdam UMC, location AMC University of Amsterdam  Amsterdam the Netherlands"},{"name":"Amsterdam Rheumatology and Immunology Center (ARC)  Amsterdam the Netherlands"},{"name":"Department of Experimental Immunology, Amsterdam UMC, location AMC University of Amsterdam  Amsterdam the Netherlands"},{"name":"Amsterdam Institute for Immunology and Infectious Diseases  Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janna E. 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