{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:08:18Z","timestamp":1772118498913,"version":"3.50.1"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,16]],"date-time":"2024-11-16T00:00:00Z","timestamp":1731715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["Grant\u2010in\u2010Aid for Scientific Research (C) (no. 22K09901)"],"award-info":[{"award-number":["Grant\u2010in\u2010Aid for Scientific Research (C) (no. 22K09901)"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Reconstitution of the thymus is essential for assessing thymic function following injury. However, the currently employed cytoreductive regimes unvaryingly affect the thymic microenvironment, thereby impeding the recovery of T lymphopoiesis. The thymic stroma is composed of epithelial and mesenchymal cells. Thymic mesenchymal cells originate from the Neural crest (NC) and mesoderm and contribute to thymus organogenesis, yet their role in thymic regeneration is unclear. In this study, using transgenic mice expressing NC\u2010specific Cre and Cre\u2010driven DT receptors, we investigated the role of NC\u2010derived mesenchymal cells in thymic regeneration following total body irradiation. We revealed that NC\u2010derived mesenchymal cells have reduced susceptibility to irradiation and induce the upregulation of hematopoietic factors that promote thymus regeneration after irradiation. Additionally, using adult thymic organ culture and renal capsule transplantation, depletion of NC\u2010derived mesenchymal cells resulted in a reduction of DN1\u2010like early T\u2010cell progenitors (ETP) and impaired thymic regeneration. Furthermore, among the numerous factors upregulated by NC\u2010derived mesenchymal cells,\n                    <jats:italic>Periostin<\/jats:italic>\n                    and\n                    <jats:italic>Flt3L<\/jats:italic>\n                    were markedly increased after irradiation and promoted abundance of DN1\u2010like ETPs during thymic reconstitution. Collectively, these findings highlight the importance of NC\u2010derived mesenchymal cells in thymic regeneration.\n                  <\/jats:p>","DOI":"10.1002\/eji.202451305","type":"journal-article","created":{"date-parts":[[2024,11,16]],"date-time":"2024-11-16T07:21:06Z","timestamp":1731741666000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Neural Crest\u2010Derived Mesenchymal Cells Support Thymic Reconstitution After Lethal Irradiation"],"prefix":"10.1002","volume":"55","author":[{"given":"Doris Narki","family":"Tetteh","sequence":"first","affiliation":[{"name":"Department of Stem Cell and Developmental Biology Mie University Graduate School of Medicine  Tsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kana","family":"Isono","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Developmental Biology Mie University Graduate School of Medicine  Tsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari","family":"Hikosaka\u2010Kuniishi","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Developmental Biology Mie University Graduate School of Medicine  Tsu Japan"},{"name":"Laboratory of Molecular Cell Biology, Graduate School of Medicine and Pharmacological Science University of Toyama  Toyama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidetoshi","family":"Yamazaki","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Developmental Biology Mie University Graduate School of Medicine  Tsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.059998"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.8.5344"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6606851"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.142.1.17"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2009.07.010"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1781"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2003.00077.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20072065"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2741\/3866"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.5.3183"},{"key":"e_1_2_10_12_1","first-page":"133","article-title":"The Role of mesenchyme in Thymus Development","volume":"251","author":"Owen J. 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