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It is less clear whether CD69\u2010negative memory T cells are also tissue\u2010resident. Here, we compare transcriptomes and T cell receptor repertoires of individual CD4 and CD8 memory T cells from paired blood and bone marrow samples from three human donors. CD69\n                    <jats:sup>\u2212<\/jats:sup>\n                    memory T cells of blood and bone marrow share transcriptionally defined clusters, characterized by signature genes and reflecting their imprinting during original activation. However, cells of related clusters from blood and bone marrow have different TCR repertoires, evidence that they represent distinct compartments of memory and indicating that the CD69\n                    <jats:sup>\u2212<\/jats:sup>\n                    memory T cells are residents of the bone marrow. Interestingly, the surface CD69\n                    <jats:sup>\u2212<\/jats:sup>\n                    memory T cells of bone marrow do transcribe the CD69 gene and express S1PR1, suggesting that they are blindfolded to the perception of the egress signal sphingosine\u20101\u2010phosphate by dimerization and internalization of CD69 and S1PR1, maintaining them in the bone marrow.\n                  <\/jats:p>","DOI":"10.1002\/eji.202451529","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T04:17:47Z","timestamp":1747369067000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Surface CD69\u2010Negative CD4 and CD8 Bone Marrow\u2010Resident Human Memory T Cells"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1402-6263","authenticated-orcid":false,"given":"Emilia","family":"Schneider Revueltas","sequence":"first","affiliation":[{"name":"Deutsches 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The human Bone Marrow May Offer an IL\u201015\u2010Dependent Survival Niche for EOMES+ Tr1\u2010Like Cells https:\/\/doi.org\/10.1002\/eji.202451644.","DOI":"10.1002\/eji.202451644"},{"key":"e_1_2_11_27_1","doi-asserted-by":"crossref","unstructured":"A.Hamann D. P.Andrew D.Jablonski\u2010Westrich B.Hoizmann andE. C.Butchert \u201cRole of a4\u2010Lntegrins in Lymphocyte Homing to Mucosal Tissues in Vivo \u201dJournal of Immunologyno.152(1994):3283\u20133293.","DOI":"10.4049\/jimmunol.152.7.3282"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abo0981"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202451018"},{"key":"e_1_2_11_30_1","article-title":"Resident Memory T Cell Development Is Associated With AP\u20101 Transcription Factor 2 Upregulation Across Anatomical Niches","author":"Smith N. 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