{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T16:09:09Z","timestamp":1785859749982,"version":"3.56.0"},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Tissue\u2010resident memory T (T\n                    <jats:sub>RM<\/jats:sub>\n                    ) cells play a key role in sustained protective immunity against\n                    <jats:italic>Bordetella pertussis<\/jats:italic>\n                    infection of the nasal mucosa. Current alum\u2010adjuvanted acellular pertussis (aP) vaccines protect against severe pertussis disease but fail to prevent nasal infection with\n                    <jats:italic>B. pertussis<\/jats:italic>\n                    . Here we demonstrate that immunization of mice with an aP vaccine failed to generate respiratory T\n                    <jats:sub>RM<\/jats:sub>\n                    cells, but did induce antigen\u2010specific CD4\n                    <jats:sup>+<\/jats:sup>\n                    Treg cells that expressed Foxp3, CD49b, PD\u20101 and LAG\u20103, and CD8\n                    <jats:sup>+<\/jats:sup>\n                    Treg cells that expressed CD122, PD\u20101, and IL\u201010.\n                    <jats:italic>B. pertussis<\/jats:italic>\n                    \u2010specific CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell lines established from aP\u2010immunized mice expressed the regulatory markers and suppressed activation of Th1 and Th17 cells. Blockade of IL\u201010 signaling during aP immunization or\n                    <jats:italic>B. pertussis<\/jats:italic>\n                    challenge promoted the induction of IL\u201017\u2010secreting CD4\n                    <jats:sup>+<\/jats:sup>\n                    T\n                    <jats:sub>RM<\/jats:sub>\n                    responses and enhanced bacterial clearance from the nose. Addition of the adjuvant LP\u2010GMP, comprising TLR2 and STING agonists, to the aP vaccine and delivery by the nasal route promoted the induction of antigen\u2010specific IL\u201017\u2010producing CD4\n                    <jats:sup>+<\/jats:sup>\n                    T\n                    <jats:sub>RM<\/jats:sub>\n                    cells and enhanced vaccine efficacy. Our findings demonstrate that aP vaccines suppress the induction of protective T\n                    <jats:sub>RM<\/jats:sub>\n                    cells in part through the induction of CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    Treg cells, which can be overcome using a potent adjuvant and delivery of the vaccine intranasally.\n                  <\/jats:p>","DOI":"10.1002\/eji.202451630","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T15:38:12Z","timestamp":1752075492000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Acellular Pertussis Vaccines Induce CD8\n                    <sup>+<\/sup>\n                    and CD4\n                    <sup>+<\/sup>\n                    Regulatory T Cells That Suppress Protective Tissue\u2010Resident Memory CD4\n                    <sup>+<\/sup>\n                    T Cells, in Part via IL\u201010"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7016-2468","authenticated-orcid":false,"given":"Caitl\u00edn","family":"N\u00ed Chasaide","sequence":"first","affiliation":[{"name":"Immune Regulation Research Group School of Biochemistry and Immunology Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7545-8253","authenticated-orcid":false,"given":"Pauline","family":"Schmitt","sequence":"additional","affiliation":[{"name":"Immune Regulation Research Group School of Biochemistry and Immunology Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9r\u00e9 K.","family":"Diallo","sequence":"additional","affiliation":[{"name":"Immune Regulation Research Group School of Biochemistry and Immunology Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Borkner","sequence":"additional","affiliation":[{"name":"Immune Regulation Research Group School of Biochemistry and Immunology Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte M.","family":"Leane","sequence":"additional","affiliation":[{"name":"Immune Regulation Research Group School of Biochemistry and Immunology Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Davoud","family":"Jazayeri","sequence":"additional","affiliation":[{"name":"Immune Regulation Research Group School of Biochemistry and Immunology Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3778-9968","authenticated-orcid":false,"given":"Sreeram","family":"Udayan","sequence":"additional","affiliation":[{"name":"Immune Regulation Research Group School of Biochemistry and Immunology Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eoin","family":"O'Neill","sequence":"additional","affiliation":[{"name":"Immune Regulation Research Group School of Biochemistry and Immunology Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy M.","family":"Curham","sequence":"additional","affiliation":[{"name":"Immune Regulation Research Group School of Biochemistry and Immunology Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry","family":"Moran","sequence":"additional","affiliation":[{"name":"Immune Regulation Research Group School of Biochemistry and Immunology Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7947-633X","authenticated-orcid":false,"given":"Mieszko M.","family":"Wilk","sequence":"additional","affiliation":[{"name":"Immune Regulation Research Group School of Biochemistry and Immunology Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3646-8222","authenticated-orcid":false,"given":"Kingston H. 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