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In this study, we assessed hundreds of virological, serological, immunological, and tissue damage biomarkers in two cohorts of patients who had experienced either mild (\n                    <jats:italic>n<\/jats:italic>\n                    = 270) or severe (\n                    <jats:italic>n<\/jats:italic>\n                    = 188) COVID\u201019, 6 to 9 months post\u2010initial infection, and in which 40% and 57.4% of patients, respectively, developed PASC. Blood analysis showed that the main differences observed in humoral, viral, and biological biomarkers were associated with the initial COVID\u201019 severity, rather than being specifically linked to PASC. However, patients with PASC displayed altered CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    memory T cell subsets, with higher cytokine\u2010secreting cells and increased terminally differentiated CD45RA\n                    <jats:sup>+<\/jats:sup>\n                    effector memory T cells (TEMRA). Elevated SARS\u2010CoV\u20102\u2010specific T cells responsive to nucleocapsid\/membrane proteins with a TEMRA phenotype were also observed. A random forest model identified these features and initial symptom duration as top variables discriminating PASC, achieving over 80% classification accuracy.\n                  <\/jats:p>","DOI":"10.1002\/eji.202551948","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T15:30:32Z","timestamp":1752075032000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Immunological and Clinical Markers of Post\u2010acute Sequelae of COVID\u201019: Insights from Mild and Severe Cases 6 Months Post\u2010infection"],"prefix":"10.1002","volume":"55","author":[{"given":"William","family":"Mouton","sequence":"first","affiliation":[{"name":"Laboratoire commun de recherche HCL\u2010bioM\u00e9rieux, Hospices Civils de Lyon  Lyon France"},{"name":"Centre International de Recherche en Infectiologie (CIRI), INSERM U1111, CNRS UMR5308, ENS Lyon Universit\u00e9 Claude Bernard Lyon 1  Lyon 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