{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:02:33Z","timestamp":1789495353696,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:00:00Z","timestamp":1753920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001706","name":"Louis-Jeantet Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001706","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000289","name":"Cancer Research UK","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells are central to targeting and eliminating cancer cells. Their function is critically supported by type 1 conventional dendritic cells (cDC1s), which both prime antigen\u2010specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells in tumour\u2010draining lymph nodes (tdLNs) and sustain primed CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells within tumours. Despite their importance, the spatiotemporal organisation of cDC1s within tumours and their diverse functional roles remain poorly understood. Here, we use scRNAseq and unbiased spatial analysis to construct a detailed map of cDC1 states and distribution within immunogenic mouse tumours during CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell\u2010mediated rejection. We reveal two distinct cDC1 activation states characterised by differential expression of genes linked to anti\u2010tumour immunity, including\n                    <jats:italic>Cxcl9<\/jats:italic>\n                    and\n                    <jats:italic>Il12b<\/jats:italic>\n                    . Strikingly,\n                    <jats:italic>Il12b<\/jats:italic>\n                    \u2010expressing cDC1s are CCR7\n                    <jats:sup>+<\/jats:sup>\n                    and enriched at tumour borders, where they closely associate with stem\u2010like TCF1\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. In contrast, CCR7\n                    <jats:sup>\u2013<\/jats:sup>\n                    <jats:italic>Cxcl9<\/jats:italic>\n                    \u2010expressing cDC1s are preferentially found within the tumour parenchyma alongside effector CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. Analysis of a published dataset of human tumours similarly reveals a spatial association between CCR7\n                    <jats:sup>+<\/jats:sup>\n                    cDC1 and stem\u2010like TCF1\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. These findings uncover a highly spatially coordinated interaction between cDC1s and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells within tumours, shedding light on the intricate cellular dynamics that underpin effective anti\u2010tumour immunity.\n                  <\/jats:p>","DOI":"10.1002\/eji.70011","type":"journal-article","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T05:25:01Z","timestamp":1754025901000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Spatial Organisation of Tumour cDC1 States Correlates with Effector and Stem\u2010Like CD8\n                    <sup>+<\/sup>\n                    T Cells Location"],"prefix":"10.1002","volume":"55","author":[{"given":"C\u00e9cile","family":"Piot","sequence":"first","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Pereira da Costa","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6169-9861","authenticated-orcid":false,"given":"Adi","family":"Biram","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos M.","family":"Minutti","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute  London UK"},{"name":"Immunoregulation Laboratory Champalimaud Research Champalimaud Centre for the Unknown  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6544-9218","authenticated-orcid":false,"given":"Kok Haw Jonathan","family":"Lim","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute  London UK"},{"name":"Division of Cancer Sciences Faculty of Biology Medicine and Health The University of Manchester; and Advanced Immunotherapy and Cell Therapy Team The Christie NHS Foundation Trust  Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Green","sequence":"additional","affiliation":[{"name":"Experimental Histopathology The Francis Crick Institute  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ania","family":"Mikolajczak","sequence":"additional","affiliation":[{"name":"Experimental Histopathology The Francis Crick Institute  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert P.","family":"Jenkins","sequence":"additional","affiliation":[{"name":"Tumour Cell Biology Laboratory The Francis Crick Institute  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Meader","sequence":"additional","affiliation":[{"name":"Experimental Histopathology The Francis Crick Institute  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Buck","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute  London UK"},{"name":"Medical Department ADM Health &amp; Wellness  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Rogers","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute  London 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