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In this study, we designed and evaluated a dendritic cell (DC)\u2010targeting multiepitope peptide\u2010based biomimetic nanovaccine for its immunogenicity and protective efficacy in a murine model. Bioinformatics tools were employed to predict and screen B\u2010 and T\u2010cell epitopes derived from the OmpW protein of\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    , followed by immunological validation. The dominant epitopes were sequentially linked using 6\u2010aminocaproic acid to synthesize a multiepitope peptide, rOmpW. Subsequently, rOmpW was encapsulated within polylactic\u2010co\u2010glycolic acid (PLGA) nanoparticles coated with neutrophil membranes (NM), and the surface was functionalized with a DC\u2010targeting peptide (DCpep) to construct the biomimetic nanovaccine, DCpep\u2010NM\u2010PLGA\u2010rOmpW. This biomimetic nanovaccine elicited robust Th1 and Th17 cellular immune responses, as well as humoral immunity, and demonstrated significant protective efficacy in a murine model of acute lethal pneumonia caused by\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    . These findings underscore the translational potential of this biomimetic nanovaccine as a prophylactic strategy against\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    infections.\n                  <\/jats:p>","DOI":"10.1002\/eji.70019","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T06:00:15Z","timestamp":1753768815000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Immunogenicity\u2010Guided Design of an\n                    <i>Acinetobacter baumanii<\/i>\n                    Vaccine"],"prefix":"10.1002","volume":"55","author":[{"given":"Chenghua","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine Pukou Hospital of Chinese Medicine Affiliated to China Pharmaceutical University  Nanjing Jiangsu China"},{"name":"Department of Pulmonary and Critical Care Medicine The Second Affiliated Hospital with 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