{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T22:33:42Z","timestamp":1786660422120,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:00:00Z","timestamp":1760918400000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["700862"],"award-info":[{"award-number":["700862"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["225315\/Z\/22\/Z"],"award-info":[{"award-number":["225315\/Z\/22\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["316213987\/C06\/B08"],"award-info":[{"award-number":["316213987\/C06\/B08"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["390713860"],"award-info":[{"award-number":["390713860"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["550453157"],"award-info":[{"award-number":["550453157"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/V006495\/1"],"award-info":[{"award-number":["BB\/V006495\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Activating immune receptors provides mechanisms for phagocytes to elicit important effector functions that promote the killing of microbes. Leukocyte immunoglobulin\u2010like receptor A5 (LILRA5), an orphan immune receptor expressed by human phagocytes and co\u2010localising with FcR\u03b3, remains poorly characterised. To address this, we developed a highly specific anti\u2010LILRA5 monoclonal antibody that has agonistic properties. We show LILRA5 expression on na\u00efve monocytes and neutrophils, and that ligation of LILRA5 stimulates ROS production. While increased\n                    <jats:italic>LILRA5<\/jats:italic>\n                    transcripts have been associated with sepsis, we also observed increased levels in patients with systemic infection but without sepsis complications. Ex vivo bacterial infection of whole blood did not alter surface LILRA5 expression, but LPS stimulation changed expression levels, indicating that surface LILRA5 expression is dynamic and likely regulated post\u2010transcriptionally, changing responses to different stimuli or over time. Soluble (s)LILRA5 was enhanced in sera from sepsis patients and in supernatants of monocytes that were LPS\u2010stimulated, indicating that shedding of LILRA5 from cell surfaces or that expression of sLILRA5 isoforms provides a mechanism to regulate surface LILRA5 expression levels. Finally, we show that altered surface LILRA5 expression influences LILRA5\u2010induced ROS production capacity. Thus, LILRA5 is a dynamically regulated activating receptor expressed on phagocytes that stimulates ROS production.\n                  <\/jats:p>","DOI":"10.1002\/eji.70079","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T04:12:02Z","timestamp":1761019922000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["LILRA5 Functions to Induce ROS Production on Innate Immune Cells"],"prefix":"10.1002","volume":"55","author":[{"given":"Zuyi","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases Centre for Bacterial Resistance Biology Section of Molecular Microbiology, Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8631-8049","authenticated-orcid":false,"given":"Matev\u017e","family":"Rumpret","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Centre for Bacterial Resistance Biology Section of Molecular Microbiology, Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5341-2066","authenticated-orcid":false,"given":"Irina","family":"Kube\u2010Golovin","sequence":"additional","affiliation":[{"name":"Department of Anatomy Medical Faculty University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mykola","family":"Lyndin","sequence":"additional","affiliation":[{"name":"Department of Anatomy Medical Faculty University of Duisburg\u2010Essen  Essen Germany"},{"name":"Department of Pathology Academic and Research Medical Institute Sumy State University  Sumy Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Solntceva","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Centre for Bacterial Resistance Biology Section of Molecular Microbiology, Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology UMC Utrecht  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Konieva","sequence":"additional","affiliation":[{"name":"Department of Anatomy Medical Faculty University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine Jena University Hospital  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian T.","family":"Press","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine Jena University Hospital  Jena Germany"},{"name":"Center for Sepsis Control and Care Jena University Hospital  Jena Germany"},{"name":"Medical Faculty Friedrich\u2010Schiller\u2010University  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie B.","family":"Floh\u00e9","sequence":"additional","affiliation":[{"name":"Department of Trauma Surgery University Hospital Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Bauer","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine Jena University Hospital  Jena Germany"},{"name":"Center for Sepsis Control and Care Jena University Hospital  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunther","family":"Wennemuth","sequence":"additional","affiliation":[{"name":"Department of Anatomy Medical Faculty University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard B","family":"Singer","sequence":"additional","affiliation":[{"name":"Department of Anatomy Medical Faculty University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4105-3737","authenticated-orcid":false,"given":"Alex J.","family":"McCarthy","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Centre for Bacterial Resistance Biology Section of Molecular Microbiology, Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,20]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-12-077917"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102005\u201003\u20101239"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.275"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.142.7.2365"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002614"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.07.006"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/jhg.2015.64"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838415"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102002\u201005\u20101432"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00857"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1501937"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0000000000003205"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753\u2010023\u201001803\u20108"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.54"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.6905"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep21780"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.141593"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12337"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900852"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ypmed.2023.107540"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.11236"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104\u2010021\u201005488\u2010w"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2013.06.034"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200839080"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40618\u2010024\u201002419\u2010x"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.662063"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020106103"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/256495a0"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.58597"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2021.103776"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.4A0415\u2010168RR"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.741484"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054\u2010020\u201003146\u20104"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9010127"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048979"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00171"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.108891"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2019.04.018"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0287"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.70079","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T06:46:00Z","timestamp":1761547560000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.70079"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":39,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1002\/eji.70079"],"URL":"https:\/\/doi.org\/10.1002\/eji.70079","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/EJI.70079\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70079\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70079\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70079\/v4\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70079\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70079\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70079\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70079\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70079\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70079\/v4\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70079\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70079\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"2025-03-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70079"}}