{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T09:20:34Z","timestamp":1788254434004,"version":"build-2803163510"},"reference-count":24,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Zinc finger E\u2010box binding protein 2 (ZEB2) is a key factor in the differentiation of na\u00efve CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells into effector and memory T cells. However, the precise regulatory role of ZEB2 in cytotoxic CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells remains unknown. Our recent DNA methylation analysis of cytomegalovirus (CMV)\u2010specific human CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells revealed two differentially methylated regions (DMRs) within the\n                    <jats:italic>ZEB2<\/jats:italic>\n                    locus. In the present study, we show that these\n                    <jats:italic>ZEB2<\/jats:italic>\n                    DMRs undergo pronounced demethylation during T cell differentiation. In particular, terminally differentiated CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells and cytotoxic CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells show an almost complete demethylation. Demethylation of the\n                    <jats:italic>ZEB2<\/jats:italic>\n                    DMRs correlates strongly with\n                    <jats:italic>ZEB2<\/jats:italic>\n                    expression in all T cell subsets. Furthermore, DNA methylation patterns remain stable during long\u2010term in vitro culture.\n                    <jats:italic>ZEB2<\/jats:italic>\n                    knockout in CD8\n                    <jats:sup>+<\/jats:sup>\n                    effector T cells results in altered gene expression profiles, affecting genes related to cell\u2013cell adhesion and impairing the cytotoxic capacity in CMV\u2010specific killing assays. Our data show that\n                    <jats:italic>ZEB2<\/jats:italic>\n                    expression contributes to the differentiation of na\u00efve CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells into effector and memory T\u00a0cells and regulates the functional properties of virus\u2010specific cytotoxic CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells.\n                  <\/jats:p>","DOI":"10.1002\/eji.70084","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:42:30Z","timestamp":1762432950000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Epigenetically Controlled\n                    <i>ZEB2<\/i>\n                    Expression Promotes the Cytotoxic Potential of CMV\u2010Specific CD8\n                    <sup>+<\/sup>\n                    T Cells"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5047-2338","authenticated-orcid":false,"given":"Varun","family":"Sasidharan Nair","sequence":"first","affiliation":[{"name":"Department Experimental Immunology Helmholtz Centre for Infection Research  Braunschweig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Yu","sequence":"additional","affiliation":[{"name":"Department Experimental Immunology Helmholtz Centre for Infection Research  Braunschweig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hosein","family":"Ahmadi","sequence":"additional","affiliation":[{"name":"Department Experimental Immunology Helmholtz Centre for Infection Research  Braunschweig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes","family":"Bonifacius","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Transplant Engineering Hannover Medical 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