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Members of the ZFP36\u2010family of RNA\u2010binding proteins, ZFP36 and ZFP36L1, regulate these functions in T cells via the regulation of mRNA stability and protein translation. We investigate the regulation of ZFP36 and ZFP36L1 expression using\n                    <jats:italic>in vitro<\/jats:italic>\n                    differentiated OT1 TCR transgenic memory\u2010like T cells. We characterise the differential kinetics and sensitivity of ZFP36 and ZFP36L1 to antigen affinity and PMA versus ionomycin stimulation. By selectively inhibiting TCR\u2010induced signalling pathways, we find that p38 MAPK, MEK1\/2, and PKC contribute to inducing both ZFP36 and ZFP36L1 expression. By contrast, inhibition of calcineurin using cyclosporin A potently inhibits ZFP36L1 expression while increasing and prolonging ZFP36 expression. The\n                    <jats:italic>Zfp36<\/jats:italic>\n                    promoter contains many binding sites for the transcription factors ELK\u20101\/4 and few binding sites for NFAT, while the\n                    <jats:italic>Zfp36l1<\/jats:italic>\n                    promoter contains many NFAT binding sites and few ELK1\/4 binding sites. Our findings suggest that the regulation of divergent transcription factors enables calcineurin to act as a signalling node that mediates the differential regulation of ZFP36 and ZFP36L1 during T cell activation.\n                  <\/jats:p>","DOI":"10.1002\/eji.70085","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T12:29:26Z","timestamp":1762777766000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Differential Regulation of TCR\u2010Induced ZFP36 and ZFP36L1 Expression by Cyclosporin A in CD8\n                    <sup>+<\/sup>\n                    T Cells"],"prefix":"10.1002","volume":"55","author":[{"given":"Marian","family":"Jones Evans","sequence":"first","affiliation":[{"name":"Immunology Programme The Babraham Institute, Babraham Research Campus  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Twm J.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Immunology Programme The Babraham Institute, Babraham Research Campus  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Zaucha","sequence":"additional","affiliation":[{"name":"Immunology Programme The Babraham Institute, Babraham Research Campus  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Petkau","sequence":"additional","affiliation":[{"name":"Immunology Programme The Babraham Institute, Babraham Research Campus  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3801-9896","authenticated-orcid":false,"given":"Martin","family":"Turner","sequence":"additional","affiliation":[{"name":"Immunology Programme The Babraham Institute, Babraham Research Campus  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2017.11.008"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.129"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010017\u20100028\u20104"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m702044200"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0021\u20109258(17)46259\u20104"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.751313"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.2.953"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m901229200"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201000892\u2010y"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.7554\/elife.33057"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010018\u20100155\u20106"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01952"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201029979\u2010x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201024132\u20106"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202350700"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","unstructured":"N. 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