{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:25:45Z","timestamp":1780446345817,"version":"3.54.1"},"reference-count":70,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Graft\u2010versus\u2010host disease is mediated by donor\u2010derived T cells reactive against the recipient's broadly expressed minor histocompatibility antigens (mHA). Regulatory T cells (Treg) have been explored as a therapeutic approach for chronic GVHD (cGVHD). The promising results from polyclonal Treg trials in this setting have led us to develop a Treg product specific for mismatched minor antigens between patient and donor (mTreg), circumventing broad immune suppression risks. HLA\u2010matched siblings of opposite sexes were used to obtain the sister's CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD25\n                    <jats:sup>hi<\/jats:sup>\n                    CD127\n                    <jats:sup>low<\/jats:sup>\n                    Treg for co\u2010culture with the respective brother's dendritic cells as a source of mismatched mHA. We have established the optimal culture conditions resulting in the highest mTreg proliferation and viability. Comprehensive phenotyping during the ex vivo selection shows PD\u20101, CTLA\u20104, CD39 and HLA\u2010DR expression. Transcriptomic analysis revealed a switch in metabolic process, and up\u2010regulation of functional Treg genes. Furthermore, mTreg possess specific and potent suppressive activity, in which there is a dependency on cell\u2010to\u2010cell contact and a role for HLA class II expression on mTreg. This protocol would allow the generation of Treg specific to an array of mHA from the recipient's healthy tissues, likely providing a directed and strong suppression of cGVHD.\n                  <\/jats:p>","DOI":"10.1002\/eji.70096","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T10:52:43Z","timestamp":1766055163000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Selection and Characterisation of Minor Histocompatibility Antigen\u2010Specific Regulatory T Cells in Fully HLA\u2010Matched Setting for GVHD Therapy"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3562-0213","authenticated-orcid":false,"given":"Carolina Paulino","family":"Pacini","sequence":"first","affiliation":[{"name":"JLacerda Lab Gulbenkian Institute for Molecular Medicine  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5534-1481","authenticated-orcid":false,"given":"Rita I.","family":"Azevedo","sequence":"additional","affiliation":[{"name":"JLacerda Lab Gulbenkian Institute for Molecular Medicine  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8911-3380","authenticated-orcid":false,"given":"Lu\u00eds","family":"Ramalhete","sequence":"additional","affiliation":[{"name":"Blood and Transplantation Center of Lisbon Instituto Portugu\u00eas do Sangue e da Transplanta\u00e7\u00e3o  Lisbon Portugal"},{"name":"NOVA Medical School Universidade NOVA De Lisboa  Lisbon Portugal"},{"name":"inova4health\u2010Advancing Precision Medicine N\u00facleo De Investiga\u00e7\u00e3o em Doen\u00e7as Renais NOVA Medical School Faculdade De Ci\u00eancias M\u00e9dicas Universidade NOVA De Lisboa  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6297-1528","authenticated-orcid":false,"given":"Hugo A. 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