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During T cell development in the thymus, thymocytes migrate from the cortex to the medulla to sequentially acquire non\u2010self\u2010reactivity and self\u2010tolerance. This cortex\u2010to\u2010medulla migration is regulated by CCR7\u2010mediated chemokine signaling. Previous studies have identified CCL21 but not CCL19 as a functional ligand for this CCR7\u2010dependent migration. CCL21 in the mouse is encoded by multiple genes, including CCL21Ser\u2010encoding\n                    <jats:italic>Ccl21a<\/jats:italic>\n                    and several CCL21Leu\u2010encoding genes, including\n                    <jats:italic>Ccl21b<\/jats:italic>\n                    . The importance of\n                    <jats:italic>Ccl21a<\/jats:italic>\n                    in thymocyte migration has been demonstrated, whereas the role of CCL21Leu\u2010encoding genes remains unclear. By producing mice specifically deficient in\n                    <jats:italic>Ccl21b<\/jats:italic>\n                    , we show that\n                    <jats:italic>Ccl21b<\/jats:italic>\n                    plays little to no role in the cortex\u2010to\u2010medulla migration of developing thymocytes. CCL21Leu\u2010encoding gene transcripts remain detectable even in the absence of\n                    <jats:italic>Ccl21b<\/jats:italic>\n                    , suggesting that\n                    <jats:italic>Ccl21b<\/jats:italic>\n                    is not a major source of CCL21Leu. We further show that the copy number of CCL21Leu\u2010encoding genes is smaller than the currently estimated copy number in a public database. These findings underscore the predominant role of\n                    <jats:italic>Ccl21a<\/jats:italic>\n                    over\n                    <jats:italic>Ccl21b<\/jats:italic>\n                    in the mouse thymus.\n                  <\/jats:p>","DOI":"10.1002\/eji.70114","type":"journal-article","created":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T10:47:31Z","timestamp":1766227651000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Ccl21a<\/i>\n                    , Rather Than\n                    <i>Ccl21b<\/i>\n                    , is Essential for Thymocyte Migration in Mouse"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0017-6957","authenticated-orcid":false,"given":"Izumi","family":"Ohigashi","sequence":"first","affiliation":[{"name":"Division of Experimental Immunology Institute of Advanced Medical Sciences Tokushima University  Tokushima Japan"},{"name":"Laboratory of Developmental Immunology Institute of Photonics and Human Health Frontier Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitomi","family":"Kyuma","sequence":"additional","affiliation":[{"name":"Division of Experimental Immunology Institute of Advanced Medical Sciences Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eri","family":"Otsu","sequence":"additional","affiliation":[{"name":"Division of Experimental Immunology Institute of Advanced Medical Sciences Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichi","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Laboratory For Embryology Institute of Advanced Medical Sciences Tokushima University  Tokushima Japan"},{"name":"Faculty of Medicine Department of Anatomy Kansai Medical University  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Takemoto","sequence":"additional","affiliation":[{"name":"Laboratory For Embryology Institute of Advanced Medical Sciences Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yousuke","family":"Takahama","sequence":"additional","affiliation":[{"name":"Thymus Biology Section, Experimental Immunology Branch National Cancer Institute, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115601"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1781"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20040643"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.7.3999"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2005.12.011"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906956106"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1203203"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/gtc.12013"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.21.13803"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.1.361"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.8.1183"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20161864"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1513"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200939921"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830210711"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270615"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.06.018"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.08.007"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI19188"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2010.03.004"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10038\u2010006\u20100391\u20108"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.92552"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1075958"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni723"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep11315"}],"container-title":["European 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