{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T11:59:27Z","timestamp":1777982367568,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    CD83 is critical for CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell selection. It regulates MHC II ubiquitination and turnover at the surface of thymic epithelial cells (TECs). The role of UBL3, a recently identified adaptor molecule for MHC II ubiquitination, is unknown in thymic selection. Here we demonstrate that UBL3 regulates MHC II in TECs and participates in CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell selection. Deleting UBL3 in CD83 loss\u2010of\u2010function mice (\n                    <jats:italic>\n                      Cd83\n                      <jats:sup>anu\/anu<\/jats:sup>\n                      Ubl3\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    ) increases MHC II on the surface of\n                    <jats:italic>\n                      Cd83\n                      <jats:sup>anu\/anu<\/jats:sup>\n                    <\/jats:italic>\n                    TECs. This increase in surface MHC II correlates with increased positive selection of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells. Analysis of\n                    <jats:italic>\n                      Cd83\n                      <jats:sup>anu\/anu<\/jats:sup>\n                    <\/jats:italic>\n                    and\n                    <jats:italic>\n                      Cd83\n                      <jats:sup>anu\/anu<\/jats:sup>\n                      Ubl3\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice identifies the CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>low<\/jats:sup>\n                    CD69\n                    <jats:sup>+<\/jats:sup>\n                    stage of positive selection as the origin of the CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell selection defect in\n                    <jats:italic>\n                      Cd83\n                      <jats:sup>anu\/anu<\/jats:sup>\n                    <\/jats:italic>\n                    mice. This stage of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell positive selection is also impacted by UBL3. The positive selection defect in the absence of CD83 also manifests as alterations in CCR7\n                    <jats:sup>+<\/jats:sup>\n                    CD4 single\u2010positive (SP) thymocytes. At the later stages of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell development, a role for UBL3 is no longer detected. In summary, through in\u2010depth phenotyping of thymocyte populations, a role for CD83 and UBL3 in regulating the early stages of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell positive selection has been identified.\n                  <\/jats:p>","DOI":"10.1002\/eji.70143","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T11:17:37Z","timestamp":1770203857000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["UBL3 Participates in the Early Stages of CD83\u2010Dependent CD4\n                    <sup>+<\/sup>\n                    T Cell Selection"],"prefix":"10.1002","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9132-8738","authenticated-orcid":false,"given":"Huw","family":"Morgan","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute The University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute The University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose A.","family":"Villadangos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute The University of Melbourne  Parkville Victoria Australia"},{"name":"Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity The University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justine D.","family":"Mintern","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute The University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010023\u201000911\u20108"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05261"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.11.001"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20160312"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20160316"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201029524\u2010w"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201039722\u20109"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092\u20108674(02)00673\u20106"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.5.2995"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20092203"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3560"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010022\u201001187\u20101"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2416"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010023\u201001584\u20100"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1501050"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3419"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.12.2301"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.09.011"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crimmu.2021.10.004"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201006197\u2010y"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.20892\/j.issn.2095\u20103941.2019.0279"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021231"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-421-0_14"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im0326s107"}],"container-title":["European 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