{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T08:07:09Z","timestamp":1781251629430,"version":"3.54.1"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:00:00Z","timestamp":1773273600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Antibodies produced by B cells aid in the recognition and clearance of pathogens and are the cornerstone of vaccination strategies. Humans produce nine different antibody isotypes, and their effector functions differ according to the type of antigen and route of exposure. Phenotypic variation between isotype\u2010switched B cell subsets is expected but not studied in detail. To obtain a molecular definition of isotype\u2010defined cell identity, we performed proteomics and transcriptomics on isotype\u2010defined populations of human naive and memory B cells (MBCs): CD27\n                    <jats:sup>\u2212<\/jats:sup>\n                    IgM\n                    <jats:sup>+<\/jats:sup>\n                    IgD\n                    <jats:sup>+<\/jats:sup>\n                    , CD27\n                    <jats:sup>+<\/jats:sup>\n                    CD38\n                    <jats:sup>lo\/\u2212<\/jats:sup>\n                    IgM\n                    <jats:sup>+<\/jats:sup>\n                    IgD\n                    <jats:sup>+<\/jats:sup>\n                    , CD27\n                    <jats:sup>+<\/jats:sup>\n                    CD38\n                    <jats:sup>lo\/\u2212<\/jats:sup>\n                    IgM\n                    <jats:sup>+<\/jats:sup>\n                    IgD\n                    <jats:sup>\u2212<\/jats:sup>\n                    , and IgA1, IgA2, IgG1, IgG2, IgG3, and IgG4 MBCs (CD27\n                    <jats:sup>+<\/jats:sup>\n                    CD38\n                    <jats:sup>lo\/\u2212<\/jats:sup>\n                    Ig\n                    <jats:sup>+<\/jats:sup>\n                    ). Combined proteome and transcriptome analysis revealed that mRNA and protein expression profiles separate isotype\u2010defined B cell subsets according to their differentiation status. mRNA and protein expression levels correlated reasonably well for many genes. IgG4\u2010switched B cells were most distinct from naive B cells in terms of mRNA as well as protein expression profiles. Besides a distinct expression profile of cytokine and Fc receptors, we identified a high expression of IgE\u2010coding mRNA in IgG4\u2010switched B cells. SDR16C5 was identified as uniquely upregulated in IgG4\u2010switched B cells. Taken together, this study highlights the distinct phenotypic profile of IgG4\u2010switched B cells.\n                  <\/jats:p>","DOI":"10.1002\/eji.70159","type":"journal-article","created":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T13:44:51Z","timestamp":1773323091000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transcriptome\u2013Proteome Analysis of Human Naive and Memory B Cell Subsets Reveals Isotype and Subclass\u2010Specific Phenotypes"],"prefix":"10.1002","volume":"56","author":[{"given":"Jana","family":"Koers","sequence":"first","affiliation":[{"name":"Sanquin Research and Landsteiner Laboratory Amsterdam University Medical Centers University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arie J.","family":"Hoogendijk","sequence":"additional","affiliation":[{"name":"Sanquin Research and Landsteiner Laboratory Amsterdam University 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Biggelaar","sequence":"additional","affiliation":[{"name":"Sanquin Research and Landsteiner Laboratory Amsterdam University Medical Centers University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9600-1312","authenticated-orcid":false,"given":"Theo","family":"Rispens","sequence":"additional","affiliation":[{"name":"Sanquin Research and Landsteiner Laboratory Amsterdam University Medical Centers University of Amsterdam  Amsterdam The Netherlands"},{"name":"Amsterdam UMC location Vrije Universiteit Amsterdam Molecular Cell Biology and Immunology  Amsterdam The Netherlands"},{"name":"Amsterdam institute for Immunology and Infectious Diseases Immunology  Amsterdam The 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