{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T11:10:01Z","timestamp":1784632201572,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:00:00Z","timestamp":1773273600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["RO1AI149341"],"award-info":[{"award-number":["RO1AI149341"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    MHC\u2010E\u2010restricted CD8 T cells are emerging as an attractive therapeutic mechanism due to their strong protective capacity and ability to respond to cells with defects in antigen processing; however, their thymic development remains poorly understood. Here, we explore the MHC ligand requirement for thymic development of T cells reactive to a self\u2010peptide (FL9) presented by mouse MHC\u2010E (Qa1\n                    <jats:sup>b<\/jats:sup>\n                    ) under conditions of deficiency in the endoplasmic reticulum aminopeptidase associated with antigen processing (ERAAP), called QFL T cells. We show that, while QFL T cells can develop in the absence of the restricting Qa1\n                    <jats:sup>b<\/jats:sup>\n                    molecule, their development was abrogated in the combined absence of classical MHC Ia and Qa1\n                    <jats:sup>b<\/jats:sup>\n                    . Interestingly, QFL thymocytes did not recognize classical MHC Ia molecules through their TCR but instead used non\u2010cognate CD8\u2010MHC Ia interactions to boost responses to MHC Ib ligands. Furthermore, we also identify an alternative ligand for the QFL TCR, the MHC Ib molecule H2\u2010T11. Our data provide evidence that both cognate and non\u2010cognate MHC interactions contribute to the development of a Qa1\n                    <jats:sup>b<\/jats:sup>\n                    \u2010specific T cell population.\n                  <\/jats:p>","DOI":"10.1002\/eji.70161","type":"journal-article","created":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T13:47:04Z","timestamp":1773323224000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Non\u2010cognate CD8 Binding to MHC I Promotes Positive Selection of an MHC\u2010E Restricted CD8 T Cell Population"],"prefix":"10.1002","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7277-5301","authenticated-orcid":false,"given":"Xiaokun","family":"Yang","sequence":"first","affiliation":[{"name":"Division of Immunology and Molecular Medicine Department of Molecular and Cell Biology University of California, Berkeley Berkeley California USA"},{"name":"Department of Immunology Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa A.","family":"Colden","sequence":"additional","affiliation":[{"name":"Division of Immunology and Molecular Medicine Department of Molecular and Cell Biology University of California, Berkeley Berkeley California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Coombs","sequence":"additional","affiliation":[{"name":"Division of Immunology and Molecular Medicine Department of Molecular and Cell Biology University of California, Berkeley Berkeley California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathya","family":"Arana","sequence":"additional","affiliation":[{"name":"Division of Immunology and Molecular Medicine Department of Molecular and Cell Biology University of California, Berkeley Berkeley California USA"},{"name":"Division of Immunology and Allergy Children's Hospital of Philadelphia  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Tran","sequence":"additional","affiliation":[{"name":"Division of Immunology and Molecular Medicine Department of Molecular and Cell Biology University of California, Berkeley Berkeley California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael Manoharan","family":"Valerio","sequence":"additional","affiliation":[{"name":"Division of Immunology and Molecular Medicine Department of Molecular and Cell Biology University of California, Berkeley Berkeley California USA"},{"name":"Center for Immunology Department of Lab Medicine and Pathology University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen A.","family":"Robey","sequence":"additional","affiliation":[{"name":"Division of Immunology and Molecular Medicine Department of Molecular and Cell Biology University of California, Berkeley Berkeley California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Coscoy","sequence":"additional","affiliation":[{"name":"Division of Immunology and Molecular Medicine Department of Molecular and Cell Biology University of California, Berkeley Berkeley California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010023\u201000911\u20108"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010020\u20100345\u2010y"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2022.101658"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010immunol\u2010032712\u2010095912"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1635"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/35869"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.10.1841"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2010.07.011"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2282"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1500654"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1381"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20091429"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112317"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1600764"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00060"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI170512"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ai.2018.09.001"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1250316"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.90466"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.13292"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.03.059"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010023\u201001644\u20105"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005688"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006384"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12519"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1237874"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe9233"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1074\u20107613(01)00252\u20107"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.10.1867"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1074\u20107613(00)80036\u20109"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2938"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.7.3145"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2018.10.006"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102164\u20109\u2010178"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302048"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/373353a0"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.1.25"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1074\u20107613(00)80247\u20102"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1074\u20107613(02)00343\u20106"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.adp5016"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.3.1661"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20221941"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.6.3707"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.70161","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/eji.70161","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.70161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T07:10:10Z","timestamp":1774422610000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.70161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1002\/eji.70161"],"URL":"https:\/\/doi.org\/10.1002\/eji.70161","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/EJI.70161\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70161\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70161\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70161\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70161\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70161\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70161\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70161\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70161\/v2\/decision1","asserted-by":"object"}]},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70161"}}