{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:29:23Z","timestamp":1782952163593,"version":"3.54.5"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T00:00:00Z","timestamp":1775260800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mesenchymal stem cells (MSC) represent the main stromal component of the bone marrow (BM) niche and are crucial to maintain hematopoietic tissue homeostasis. MSC exhibits extraordinary and multiple properties. In terms of expanding potential and differentiation capacity, Wharton jelly MSC (WJ\u2010MSC), derived from the umbilical cord, was described as being greater and more performing than MSC from BM or other sources. WJ\u2010MSC mimics the hematopoietic niche and supports hematopoietic stem cells (HSC) expansion ex vivo. This study aimed to evaluate the effects of human WJ\u2010MSC cocultured with HSC in a B\u2010cell differentiation protocol. Remarkably, results highlight WJ\u2010MSC use as a preferable feeder layer to efficiently support HSC commitment toward the B\u2010lineage. Over 11 days of HSC coculture with WJ\u2010MSC, B\u2010cell genes (\n                    <jats:italic>E2A<\/jats:italic>\n                    ,\n                    <jats:italic>RAG1<\/jats:italic>\n                    ,\n                    <jats:italic>RAG2<\/jats:italic>\n                    , etc.) expression patterns and B\u2010cell markers (CD19, immunoglobulin chain, etc.) acquisition were evidenced. WJ\u2010MSc were also able to unlock the B\u2010lineage differentiation blockade of the acute lymphoblastic leukemia cell line Nalm16. This model might provide a new strategy to support ex vivo B\u2010cell differentiation using the powerful properties of WJ\u2010MSC. This study implements a new approach to improve understanding of B\u2010leukemogenesis and B\u2010cell acute lymphoblastic leukemia (B\u2010ALL) pathophysiology.\n                  <\/jats:p>","DOI":"10.1002\/eji.70186","type":"journal-article","created":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T09:06:17Z","timestamp":1775293577000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["B\u2010Cell Differentiation of Human Hematopoietic Progenitors Is Efficiently Supported by Wharton Jelly\u2010Derived Mesenchymal Stem Cells"],"prefix":"10.1002","volume":"56","author":[{"given":"Louison","family":"Collet","sequence":"first","affiliation":[{"name":"Laboratoire HEMATIM UR4666 Universit\u00e9 Picardie Jules Verne  Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hakim","family":"Ouled\u2010Haddou","sequence":"additional","affiliation":[{"name":"Laboratoire HEMATIM UR4666 Universit\u00e9 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