{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T09:31:25Z","timestamp":1786699885334,"version":"build-2736575974"},"reference-count":68,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Y\u2010box binding protein 1 (YB\u20101) is a multifunctional RNA\u2010 and DNA\u2010binding protein with broad regulatory functions in gene expression, particularly at the posttranscriptional level. Here, we demonstrate that conditional deletion of YB\u20101 at the double\u2010positive (DP) thymocyte stage causes an \u223c80% reduction of invariant natural killer T (\n                    <jats:italic>i<\/jats:italic>\n                    NKT) cells in thymus, spleen, and liver, evident already in day\u201014 neonates and persisting into adulthood. Our data reveal CD44\n                    <jats:sup>\u2212<\/jats:sup>\n                    NK1.1\n                    <jats:sup>\u2212<\/jats:sup>\n                    stage 1 accumulation and a selective loss of CD44\n                    <jats:sup>+<\/jats:sup>\n                    NK1.1\n                    <jats:sup>+<\/jats:sup>\n                    stage 3\n                    <jats:italic>i<\/jats:italic>\n                    NKT cells, indicating a postselection maturation defect. All\n                    <jats:italic>i<\/jats:italic>\n                    NKT cell subsets (\n                    <jats:italic>i<\/jats:italic>\n                    NKT1,\n                    <jats:italic>i<\/jats:italic>\n                    NKT2,\n                    <jats:italic>i<\/jats:italic>\n                    NKT17) were reduced, with thymic\n                    <jats:italic>i<\/jats:italic>\n                    NKT1 and splenic\n                    <jats:italic>i<\/jats:italic>\n                    NKT17 cells most severely affected. PMA\/ionomycin\u2010stimulated YB\u20101\u2010deficient\n                    <jats:italic>i<\/jats:italic>\n                    NKT cells showed preserved IFN\u2010\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    \/IL\u20104\n                    <jats:sup>+<\/jats:sup>\n                    frequencies but reduced per\u2010cell cytokine production and a loss of IL\u201017 production. Interestingly, YB\u20101\n                    <jats:sup>KO<\/jats:sup>\n                    DP thymocytes showed increased CD1d levels, suggesting increased TCR signal strength in the thymus of YB\u20101\u2010deficient mice. Whereas CD5 levels were elevated, basal Nur77, ICOS, and CD122 (IL\u201015R\u03b2) expression were reduced in\n                    <jats:italic>i<\/jats:italic>\n                    NKT cells. Furthermore, apoptosis was increased, particularly at\n                    <jats:italic>i<\/jats:italic>\n                    NKT stages 2\u20133. Together, these findings identify YB\u20101 as a central regulator of\n                    <jats:italic>i<\/jats:italic>\n                    NKT cell development that integrates TCR, co\u2010stimulatory, and IL\u201015 signaling to ensure postselection\n                    <jats:italic>i<\/jats:italic>\n                    NKT cell maturation, effector subset specification, and survival.\n                  <\/jats:p>","DOI":"10.1002\/eji.70201","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T12:36:50Z","timestamp":1779194210000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The RNA\u2010 and DNA\u2010Binding Protein Y\u2010Box Binding Protein 1 (YB\u20101) Regulates\n                    <i>i<\/i>\n                    NKT Cell Development"],"prefix":"10.1002","volume":"56","author":[{"given":"Silvia","family":"Schulze","sequence":"first","affiliation":[{"name":"Institute of Clinical Immunology and Cell Therapeutics Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"},{"name":"Health Campus Immunology, Infectiology and Inflammation (GCI<sup>3<\/sup>), Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Knop","sequence":"additional","affiliation":[{"name":"Institute of Clinical Immunology and Cell Therapeutics Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"},{"name":"Health Campus Immunology, Infectiology and Inflammation (GCI<sup>3<\/sup>), Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nouria","family":"Jantz\u2010Naeem","sequence":"additional","affiliation":[{"name":"Institute of Clinical Immunology and Cell Therapeutics Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"},{"name":"Health Campus Immunology, Infectiology and Inflammation (GCI<sup>3<\/sup>), Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladyslava","family":"Dovhan","sequence":"additional","affiliation":[{"name":"Institute of Clinical Immunology and Cell Therapeutics Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"},{"name":"Health Campus Immunology, Infectiology and Inflammation (GCI<sup>3<\/sup>), Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Fricke","sequence":"additional","affiliation":[{"name":"Institute of Clinical Immunology and Cell Therapeutics Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"},{"name":"Health Campus Immunology, Infectiology and Inflammation (GCI<sup>3<\/sup>), Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"},{"name":"Fraunhofer Institute for Cell Therapy and Immunology Perlickstr  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sascha","family":"Kahlfu\u00df","sequence":"additional","affiliation":[{"name":"Institute of Clinical Immunology and Cell Therapeutics Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"},{"name":"Health Campus Immunology, Infectiology and Inflammation (GCI<sup>3<\/sup>), Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"},{"name":"Institute of Medical Microbiology and Hospital Hygiene, Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"},{"name":"Center For Health and Medical Prevention (CHaMP) Otto\u2010von\u2010Guericke\u2010University  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Sch\u00fcler","sequence":"additional","affiliation":[{"name":"Institute of Clinical Immunology and Cell Therapeutics Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"},{"name":"Health Campus Immunology, Infectiology and Inflammation (GCI<sup>3<\/sup>), Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Bommhardt","sequence":"additional","affiliation":[{"name":"Institute of Clinical Immunology and Cell Therapeutics Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg Germany"},{"name":"Health Campus Immunology, Infectiology and Inflammation (GCI<sup>3<\/sup>), Medical Faculty Otto\u2010von\u2010Guericke\u2010University Magdeburg  Magdeburg 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