{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T06:29:57Z","timestamp":1786688997511,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The activating receptor NKp30 is important in NK cell killing of cancer cells. Here, we demonstrated that a pair of splice signals in the Ig domain exon of human NKp30 is largely conserved among primates and placental mammals and produces an alternatively spliced NKp30 ectodomain (NKp30\u2010S) with an in\u2010frame, nonartefactual deletion of 25 amino acid residues. Transfection yielded NKp30\u2010S bands in NKL cells but not in 293T cells, suggesting that the splicing mechanism is cell\u2010specific. Molecular modeling indicated that the overall folding of NKp30\u2010S is maintained compared with NKp30. NKp30\u2010S was expressed at the cell surface but did not bind to the NKp30 ligand B7H6 in soluble fusion protein or reporter cell assays. Single\u2010cell RNA sequencing showed that NKp30\u2010S transcription was not restricted to major NK cell subsets but suggested that a small fraction of blood NK cells expressed only the NKp30\u2010S splice variant. The ratio of NKp30\u2010S to full\u2010length transcript was increased after activation of blood NK cells with IL\u20102 or IL\u201015 or crosslinking with anti\u2010CD16 antibody, suggesting that this unique splicing mechanism, not seen in other Ig superfamily proteins, is regulated and may play a role in modulating NK cell responsiveness toward B7H6\n                    <jats:sup>+<\/jats:sup>\n                    cancer cells in vivo.\n                  <\/jats:p>","DOI":"10.1002\/eji.70215","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:27:42Z","timestamp":1780471662000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Unique Intraexonic Splicing Mechanism in the Immunoglobulin Domain of NKp30 Produces a Surface\u2010Expressed Receptor With Altered Ligand Specificity"],"prefix":"10.1002","volume":"56","author":[{"given":"Elisabeth G.","family":"Bj\u00f8rnsen","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine Institute of Basic Medical Sciences University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen Sofie","family":"Pete","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Institute of Basic Medical Sciences University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Institute of Basic Medical Sciences University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marit","family":"Inngjerdingen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Division of Laboratory Medicine Institute of Clinical Medicine University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatyana","family":"Sandalova","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory Department of Medicine Solna Karolinska Institutet and Division of Infectious Diseases Karolinska University Hospital  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4421-0766","authenticated-orcid":false,"given":"Anne","family":"Spurkland","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Institute of Basic Medical Sciences University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Daws","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Institute of Basic Medical Sciences University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adnane","family":"Achour","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory Department of Medicine Solna Karolinska Institutet and Division of Infectious Diseases Karolinska University Hospital  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9640-1954","authenticated-orcid":false,"given":"Andreas","family":"Lossius","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Institute of Basic Medical Sciences University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0216-6739","authenticated-orcid":false,"given":"Erik","family":"Dissen","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Institute of Basic Medical Sciences University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.10.1505"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.162.8.4745"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00909"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1190"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002195"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/515579"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201003014\u20104"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.4.2392"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.603464"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.10.010"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.483602"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20090681"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201847746"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588\u2010021\u201000889\u2010w"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02879"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2024.105459"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102005\u201008\u2010027979"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2015.1041701"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.adj7970"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002510000192"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00369"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2366"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10183"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa2327"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2016.1154251"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.796072"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.06.040188.002121"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1044\u20105323(03)00047\u20102"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1100622108"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20102548"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12071998"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1201983"},{"key":"e_1_2_10_34_1","first-page":"1231","article-title":"The Establishment of a Cell Line of Human Hormone\u2010Synthesizing Trophoblastic Cells in Vitro","volume":"28","author":"Pattillo R. A.","year":"1968","journal-title":"Cancer Research"},{"key":"e_1_2_10_35_1","first-page":"406","article-title":"Characterization of a Cell Line, NKL, Derived From an Aggressive Human Natural Killer Cell Leukemia","volume":"24","author":"Robertson M. J.","year":"1996","journal-title":"Experimental Hematology"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521\u20104141(2001010)31:10<2926::aid\u2010immu2926>3.0.co;2\u20102"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010024\u201007487\u2010w"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010022\u201001311\u20104"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giy059"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4197"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky955"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.048"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1754"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010024\u201001883\u20100"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0730640100"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022842"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1388018"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011149"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/7403"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102407\u20109\u2010186"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI45816"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/6248590"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.70215","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/eji.70215","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.70215","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T05:56:32Z","timestamp":1786686992000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.70215"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":53,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/eji.70215"],"URL":"https:\/\/doi.org\/10.1002\/eji.70215","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/EJI.70215\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70215\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70215\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70215\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70215\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70215\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70215\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70215\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.70215\/v2\/response1","asserted-by":"object"}]},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2025-09-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70215"}}