{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T09:24:08Z","timestamp":1786958648236,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Chimeric antigen receptor (CAR)\u2010T\u2010based strategies target the B\u2010cell maturation antigen (BCMA) expressed on the surface of malignant plasma cells in patients with multiple myeloma (MM). Idecabtagene vicleucel (ide\u2010cel), an anti\u2010BCMA CAR\u2010T therapy, has shown promising results in the treatment of relapsed or refractory multiple myeloma (R\/RMM). Notably, the extent of CAR\u2010T cell expansion correlates with objective response rates and complete responses, indicating that monitoring CAR\u2010T cells is critical for predicting clinical outcomes in these patients. Although several tools are available to monitor CAR\u2010T cells in patient blood samples, they remain costly, prompting us to develop novel fluorescent and soluble BCMA reagents for CAR\u2010T cell labeling. BCMA is a type III transmembrane protein, and we found that a recombinant construct consisting of the BCMA extracellular domain fused to green fluorescent protein (GFP) failed to traffic through the Golgi apparatus and was not secreted. Incorporation of the interferon\u2010\u03b12 signal peptide restored intracellular trafficking and enabled efficient secretion of soluble BCMA\u2010GFP and BCMA\u2010mCherry fusion proteins. These reagents specifically labeled anti\u2010BCMA CAR\u2010T cells. Overall, our results establish a cost\u2010effective method to generate soluble BCMA probes and provide accessible tools for monitoring CAR\u2010T cells in translational settings.<\/jats:p>","DOI":"10.1002\/eji.70251","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T06:15:51Z","timestamp":1785392151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineering Soluble Recombinant BCMA for Ide\u2010Cel Labeling"],"prefix":"10.1002","volume":"56","author":[{"given":"Ly Phuong Vy","family":"Nguyen","sequence":"first","affiliation":[{"name":"INSERM U1262, UMR CNRS 7276 Universit\u00e9 de Limoges  Limoges France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Oblet","sequence":"additional","affiliation":[{"name":"INSERM U1262, UMR CNRS 7276 Universit\u00e9 de Limoges  Limoges 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