{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:04:47Z","timestamp":1790661887606,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Vaccines delivered via the nasal route have attracted increasing attention for their capacity to engage the mucosal immune system at the primary site of pathogen entry. However, the early innate mechanisms that shape local immune responses following nasal immunisation remain poorly defined. Here, we identified previously uncharacterised tissue\u2010derived mediators induced during vaccination. Using an intranasally administered whole\u2010cell pertussis (wP) vaccine that recapitulates key features of\n                    <jats:italic>Bordetella pertussis<\/jats:italic>\n                    infection and elicits long\u2010lasting immunity, we show that nasal delivery triggers neutrophil recruitment along with a marked upregulation of secretory leukocyte protease inhibitor (SLPI). Employing SLPI\u2010 and neutrophil\u2010deficient mouse models, we demonstrate that SLPI and neutrophils influence the production of key mediators associated with coagulation (PAI\u20101) and tissue remodelling (MMP\u20103 and MMP\u20109) within the airway. Notably, neutrophils also modulate early local total IgA release. Together, these findings suggest a coordinated and context\u2010dependent interplay between cellular and soluble innate components that shape early mucosal immune responses. Furthermore, identified mediators may represent candidate biomarkers in the development of intranasal vaccines against\n                    <jats:italic>B. pertussis<\/jats:italic>\n                    and other pathogens.\n                  <\/jats:p>","DOI":"10.1002\/eji.70282","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T07:20:31Z","timestamp":1789543231000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Loss of Neutrophils and SLPI Reshapes Early Mucosal Inflammatory Responses During Intranasal Vaccination"],"prefix":"10.1002","volume":"56","author":[{"given":"Oktawia","family":"Osiecka","sequence":"first","affiliation":[{"name":"Department of Immunology Faculty of Biochemistry, Biophysics, and Biotechnology Jagiellonian University  Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariia","family":"Tyshchenko","sequence":"additional","affiliation":[{"name":"Department of Immunology Faculty of Biochemistry, Biophysics, and Biotechnology Jagiellonian University  Krak\u00f3w Poland"},{"name":"Doctoral School of Exact and Natural Sciences Jagiellonian University  Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Sinkevich","sequence":"additional","affiliation":[{"name":"Department of Immunology Faculty of Biochemistry, Biophysics, and Biotechnology Jagiellonian University  Krak\u00f3w Poland"},{"name":"Doctoral School of Exact and Natural Sciences Jagiellonian University  Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Poca\u0142u\u0144","sequence":"additional","affiliation":[{"name":"Department of Immunology Faculty of Biochemistry, Biophysics, and Biotechnology Jagiellonian University  Krak\u00f3w Poland"},{"name":"Doctoral School of Exact and Natural Sciences Jagiellonian University  Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Cichy","sequence":"additional","affiliation":[{"name":"Department of Immunology Faculty of Biochemistry, Biophysics, and Biotechnology Jagiellonian University  Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub M.","family":"Kwiecinski","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Biochemistry, Biophysics, and Biotechnology Jagiellonian University  Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Oleszycka","sequence":"additional","affiliation":[{"name":"Department of Immunology Faculty of Biochemistry, Biophysics, and Biotechnology Jagiellonian University  Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mieszko M.","family":"Wilk","sequence":"additional","affiliation":[{"name":"Department of Immunology Faculty of Biochemistry, Biophysics, and Biotechnology Jagiellonian University  Krak\u00f3w 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