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The complexes were derived from ligand isomers with 3\u2010methylpentyl (H<jats:sub>2<\/jats:sub>L<jats:sup>2<\/jats:sup>) and cyclohexyl (H<jats:sub>2<\/jats:sub>L<jats:sup>3<\/jats:sup>) backbone substituents, which each yield a pair of linkage isomers. The thermodynamic products CuL<jats:sup>2a\/3a<\/jats:sup> have two imino N and two S donors resulting in three five\u2010member chelate rings (555 isomers). The kinetic isomers CuL<jats:sup>2b\/3b<\/jats:sup> have one imino and one hydrazino N donor and two S donors resulting in four\u2010, six\u2010, and five\u2010member rings (465 isomers). The 555 isomers have more accessible Cu<jats:sup>II\/I<\/jats:sup> potentials (E<jats:sub>1\/2<\/jats:sub>=\u2212811\/\u2212768\u2005mV vs. ferrocenium\/ferrocene) and lower energy charge transfer bands than their 465 counterparts (E<jats:sub>1\/2<\/jats:sub>=\u2212923\/\u2010854\u2005mV). Antiproliferation activities were evaluated against the lung adenocarcinoma cell line (A549) and nonmalignant lung fibroblast cell line (IMR\u201090) using the MTT assay. CuL<jats:sup>2a<\/jats:sup> was potent (<jats:sup>A549<\/jats:sup>EC<jats:sub>50<\/jats:sub>=0.080\u2005\u03bcM) and selective (<jats:sup>IMR\u201090<\/jats:sup>EC<jats:sub>50<\/jats:sub>\/<jats:sup>A549<\/jats:sup>EC<jats:sub>50<\/jats:sub>=25) for A549. Its linkage isomer CuL<jats:sup>2b<\/jats:sup> had equivalent A549 activity, but lower selectivity (<jats:sup>IMR\u201090<\/jats:sup>EC<jats:sub>50<\/jats:sub>\/<jats:sup>A549<\/jats:sup>EC<jats:sub>50<\/jats:sub>=12.5). The isomers CuL<jats:sup>3a<\/jats:sup> and CuL<jats:sup>3b<\/jats:sup> were less potent with <jats:sup>A549<\/jats:sup>EC<jats:sub>50<\/jats:sub>\u00a0values of 1.9 and 0.19\u2005M and less selective with <jats:sup>IMR\u201090<\/jats:sup>EC<jats:sub>50<\/jats:sub>\/<jats:sup>A549<\/jats:sup>EC<jats:sub>50<\/jats:sub> ratios of 2.3 and 2.65, respectively. There was no correlation between reduction potential and A549 antiproliferation activity\/selectivity.<\/jats:p>","DOI":"10.1002\/ejic.202300447","type":"journal-article","created":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T13:58:16Z","timestamp":1694699896000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ligand and Linkage Isomers of Bis(ethylthiocarbamato) Copper Complexes with Cyclic C<sub>6<\/sub>H<sub>8<\/sub> Backbone Substituents: Synthesis, Characterization, and Antiproliferation Activity"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8073-9320","authenticated-orcid":false,"given":"Kritika","family":"Bajaj","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Louisville  40292 Louisville KY United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1751-8597","authenticated-orcid":false,"given":"Sarah A.","family":"Andres","sequence":"additional","affiliation":[{"name":"Department of Medicine and Brown Cancer Center University of Louisville  40202 Louisville KY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8638-2465","authenticated-orcid":false,"given":"Dillon T.","family":"Hofsommer","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Louisville  40292 Louisville KY United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6674-4080","authenticated-orcid":false,"given":"Okolocha","family":"Chekwube Michael","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Louisville  40292 Louisville KY United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2724-7252","authenticated-orcid":false,"given":"Mark S.","family":"Mashuta","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Louisville  40292 Louisville KY United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4622-4152","authenticated-orcid":false,"given":"Paula J.","family":"Bates","sequence":"additional","affiliation":[{"name":"Department of Medicine and Brown Cancer Center University of Louisville  40202 Louisville KY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8653-5388","authenticated-orcid":false,"given":"Robert M.","family":"Buchanan","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Louisville  40292 Louisville KY United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4889-2645","authenticated-orcid":false,"given":"Craig A.","family":"Grapperhaus","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Louisville  40292 Louisville KY United States"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,9,29]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/cber.190704001117"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0010-8545(00)80322-7"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0010-8545(97)00047-7"},{"key":"e_1_2_9_4_1","first-page":"1597","volume":"95","author":"Hazari A.","year":"2018","journal-title":"J. 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