{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:48:18Z","timestamp":1790614098927,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,1,19]],"date-time":"2018-01-19T00:00:00Z","timestamp":1516320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Pain"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The mechanisms underlying sex\u2010based differences in pain and analgesia are poorly understood. In this study, we investigated gene expression changes in trigeminal ganglia (\n                      <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>\n                      ) of male and female rats exposed to infraorbital nerve chronic constriction injury (IoN\u2010\n                      <jats:styled-content style=\"fixed-case\">CCI<\/jats:styled-content>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Somatosensory assessments were performed prior to IoN\u2010\n                      <jats:styled-content style=\"fixed-case\">CCI<\/jats:styled-content>\n                      and at selected time points postsurgery. Selected gene expression changes were examined with real\u2010time quantitative polymerase chain reaction (\n                      <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\n                      \u2010\n                      <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                      ) in ipsilateral\n                      <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>\n                      at 21\u00a0days postsurgery.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Rats exposed to IoN\u2010\n                      <jats:styled-content style=\"fixed-case\">CCI<\/jats:styled-content>\n                      developed significant mechanical allodynia and hyperalgesia on days 19 and 21 postsurgery. During this period, females developed significantly more allodynia but not hyperalgesia compared to males. At 21\u00a0days postsurgery, expression levels of 44 of the 84 investigated pain\u2010related genes in ipsilateral\n                      <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>\n                      were significantly regulated relative to na\u00efve rats in either sex.\n                      <jats:italic>Csf1 and Cx3cr1<\/jats:italic>\n                      were up\u2010regulated in both sexes, but the magnitude of regulation was significantly higher in females (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.02 and\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.001, respectively).\n                      <jats:italic>Htr1a<\/jats:italic>\n                      and\n                      <jats:italic>Scn9a<\/jats:italic>\n                      were down\u2010regulated in both sexes, but the down\u2010regulation was significantly more pronounced in males (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.04 and\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.02, respectively). Additionally,\n                      <jats:italic>Cck, Il1a, Pla2g1b and Tnf genes<\/jats:italic>\n                      were significantly regulated in females but not in males, and\n                      <jats:italic>Chrna4<\/jats:italic>\n                      gene was significantly down\u2010regulated in males but not in females.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings suggest sex\u2010dependent gene regulation in response to nerve injury, which may contribute to sex dimorphism of trigeminal neuropathic pain. Further studies are needed to establish gene expression changes over time and correlate these with hormonal and other physiological parameters in male and female.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Significance<\/jats:title>\n                    <jats:p>We present novel sex\u2010specific transcriptional regulation in trigeminal ganglia that may contribute to male\u2010\/female\u2010based differences in trigeminal neuropathic pain. These findings are expected to open new research horizons, particularly in male versus female targeted therapeutic regimens.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ejp.1174","type":"journal-article","created":{"date-parts":[[2018,1,19]],"date-time":"2018-01-19T06:51:01Z","timestamp":1516344661000},"page":"875-888","source":"Crossref","is-referenced-by-count":34,"title":["Differential gene expression in trigeminal ganglia of male and female rats following chronic constriction of the infraorbital nerve"],"prefix":"10.1002","volume":"22","author":[{"given":"O.A.","family":"Korczeniewska","sequence":"first","affiliation":[{"name":"Department of Diagnostic Sciences Rutgers School of Dental Medicine Rutgers The State University of New Jersey Newark NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Husain","sequence":"additional","affiliation":[{"name":"The Genomics Center New Jersey Medical School Rutgers The State University of New Jersey Newark NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Khan","sequence":"additional","affiliation":[{"name":"Eastman Institute for Oral Health University of Rochester Medical Center Rochester NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Eliav","sequence":"additional","affiliation":[{"name":"Eastman Institute for Oral Health University of Rochester Medical Center Rochester NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Soteropoulos","sequence":"additional","affiliation":[{"name":"The Genomics Center New Jersey Medical School Rutgers The State University of New Jersey Newark NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Benoliel","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Sciences Rutgers School of Dental Medicine Rutgers The State University of New Jersey Newark NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2010.03.021"},{"key":"e_1_2_7_3_1","unstructured":"Arikawa E. 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