{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:25:14Z","timestamp":1787239514528,"version":"build-2736575974"},"reference-count":59,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T00:00:00Z","timestamp":1648425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81800666"],"award-info":[{"award-number":["81800666"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170786"],"award-info":[{"award-number":["82170786"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670688"],"award-info":[{"award-number":["81670688"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82100816"],"award-info":[{"award-number":["82100816"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2016A030313192"],"award-info":[{"award-number":["2016A030313192"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2021A1515010354"],"award-info":[{"award-number":["2021A1515010354"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2018A0303130330"],"award-info":[{"award-number":["2018A0303130330"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["19ykpy33"],"award-info":[{"award-number":["19ykpy33"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Pain"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Bladder pain syndrome\/interstitial cystitis (BPS\/IC) is a refractory disease accompanied by bladder\u2010related pain and hyperactivity. Studies have shown that the translocator protein (TSPO) modulates neuroinflammation and central sensitisation associated with pain. Moreover, we previously demonstrated that brain\u2010derived neurotrophic factor (BDNF) regulates neuroinflammation and mechanical allodynia in cyclophosphamide (CYP)\u2010induced cystitis through activation of glial cells. Here, we aimed to explore whether activation of TSPO attenuates mechanical allodynia and bladder dysfunction by regulating BDNF induced neuroinflammation in a CYP\u2010induced cystitis model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Injection of CYP was performed to form a rat model of BPS\/IC. The expression of TSPO was regulated by intrathecal injection of the TSPO agonist Ro5\u20104864. The von Frey filament test was applied to evaluate suprapubic allodynia. Bladder function was assessed using filling cystometry. Western blotting was used to detect the expression of TSPO, BDNF, GFAP, Iba\u20101,\n                      <jats:italic>p<\/jats:italic>\n                      \u2010p38,\n                      <jats:italic>p<\/jats:italic>\n                      \u2010JNK, TNF\u2010\u03b1, and IL\u20101\u03b2, and double immunofluorescence was performed to localise TSPO in the L6\u2013S1\u00a0spinal dorsal horn (SDH).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      TSPO was activated in the SDH after CYP injection and was primarily colocalised with astrocytes. Ro5\u20104864 reversed mechanical allodynia and bladder dysfunction induced by CYP. Moreover, the upregulation of BDNF and activation of astrocytes and microglia was suppressed by Ro5\u20104864, resulting in downregulation of\n                      <jats:italic>p<\/jats:italic>\n                      \u2010p38,\n                      <jats:italic>p<\/jats:italic>\n                      \u2010JNK, TNF\u2010\u03b1, and IL\u20101\u03b2.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Ro5\u20104864 alleviated mechanical allodynia and bladder dysfunction in the CYP model, possibly by inhibiting the elevation of BDNF and consequent activation of astrocytes and microglia induced neuroinflammation. TSPO may be a potential target for the treatment of BPS\/IC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Significance<\/jats:title>\n                    <jats:p>This study examined the mechanism underlying the ability of the translocator protein to modulate bladder pain syndrome\/interstitial cystitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ejp.1942","type":"journal-article","created":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T03:59:05Z","timestamp":1647403145000},"page":"1234-1244","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Activation of translocator protein alleviates mechanical allodynia and bladder dysfunction in cyclophosphamide\u2010induced cystitis through repression of BDNF\u2010mediated neuroinflammation"],"prefix":"10.1002","volume":"26","author":[{"given":"Yong","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Urology the Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minzhi","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation the Third Affiliated Hospital and Lingnan Hospital of the Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology the Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailun","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department of Urology the Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou 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