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This study aimed to evaluate the temporal expression of circulating miRNAs in the plasma of healthy volunteers as a marker for epigenetic changes before and after an acute, experimental, pain provocation by intramuscular hypertonic saline injection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty volunteers were randomly allocated into two groups and received either hypertonic (pain) or isotonic (control) saline injection in the first dorsal interosseous muscle of their dominant hand. Pain intensity was continuously recorded for 20\u00a0minutes after injection on a VAS scale from 0 to 100 (0 indicates no pain and 100 the worst imaginable pain). Blood samples were taken at baseline, 30\u00a0minutes, 3\u00a0hours, and 24\u00a0hours post\u2010injection, and plasma was separated. MiRNA extracts were used for RNA sequencing with the Illumina NextSeq platform. MiRNA transcripts were compared between the pain and the no\u2010pain, control group at every time point. Significant differences were considered when folds were\u2009&gt;2 and the False Discovery Rate was<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.05.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 30\u00a0minutes, 4 miRNAs were significantly altered in the pain group compared to controls, which increased to 24 after 3\u2009hours and to 42 after 24\u00a0hours from baseline (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.0001). Two miRNAs were consistently upregulated throughout the experiment. Enrichment analysis showed significant miRNAs involved in brain perception of pain, brain signalling and response to stimuli.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This exploratory study is the first to report on the temporal expression of circulating miRNAs after an acute, human experimental muscle pain model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance<\/jats:title><jats:p>This exploratory study evaluated the temporal profile of circulating miRNAs in the plasma of healthy subjects after acute experimental pain. Several miRNAs were altered in subjects at the times of follow\u2010up after the acute pain model when compared to controls. MiRNAs previously associated with pain processes were altered in the pain group. Our results, by showing the fast and prolonged modifications of miRNA elicited by the acute experimental pain model, add new perspectives to the topic of epigenetics and pain.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ejp.2062","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T12:41:09Z","timestamp":1669898469000},"page":"366-377","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The temporal expression of circulating microRNAs after acute experimental pain in humans"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1331-129X","authenticated-orcid":false,"given":"Rocco","family":"Giordano","sequence":"first","affiliation":[{"name":"Center for Neuroplasticity and Pain (CNAP), SMI, Aalborg University Department of Health Science and Technology, Faculty of Medicine Aalborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5096-2588","authenticated-orcid":false,"given":"Maria Carla","family":"Gerra","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Life science, and Environmental Sustainability University of Parma Parma Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7134-9942","authenticated-orcid":false,"given":"Hiroai","family":"Okutani","sequence":"additional","affiliation":[{"name":"Center for Neuroplasticity and Pain (CNAP), SMI, Aalborg University Department of Health Science and Technology, Faculty of Medicine Aalborg Denmark"},{"name":"Department of Anesthesiology and Pain Medicine Hyogo College of Medicine Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6548-2459","authenticated-orcid":false,"given":"Silvia","family":"Lo Vecchio","sequence":"additional","affiliation":[{"name":"Center for Neuroplasticity and Pain (CNAP), SMI, Aalborg University Department of Health Science and Technology, Faculty of Medicine Aalborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9888-1955","authenticated-orcid":false,"given":"Allan","family":"Stensballe","sequence":"additional","affiliation":[{"name":"Department of Health Science and Technology Aalborg University Aalborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4506-000X","authenticated-orcid":false,"given":"Kristian Kj\u00e6r\u2010Staal","family":"Petersen","sequence":"additional","affiliation":[{"name":"Center for Neuroplasticity and Pain (CNAP), SMI, Aalborg University Department of Health Science and Technology, Faculty of Medicine Aalborg Denmark"},{"name":"Center for Mathematical Modeling of Knee Osteoarthritis (MathKOA), Aalborg University Department of Material and Production, Faculty of Engineering and Science Aalborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Arendt\u2010Nielsen","sequence":"additional","affiliation":[{"name":"Center for Neuroplasticity and Pain (CNAP), SMI, Aalborg University Department of Health Science and Technology, Faculty of Medicine Aalborg Denmark"},{"name":"Department of Gastroenterology &amp; 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