{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:27:59Z","timestamp":1790422079559,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,12,8]],"date-time":"2014-12-08T00:00:00Z","timestamp":1417996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Pain"],"published-print":{"date-parts":[[2015,9]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>The anterior pretectal nucleus (APtN<\/jats:styled-content>) activates descending mechanisms of pain control. This study evaluated whether the APtN<\/jats:styled-content> also controls neuropathic pain in rats.<\/jats:p><\/jats:sec>Methods<\/jats:title>The hypersensitivity to mechanical stimulation with an electronic v<\/jats:styled-content>on F<\/jats:styled-content>rey apparatus and the number of F<\/jats:styled-content>os\u2010immunoreactive (F<\/jats:styled-content>os\u2010ir) neurons in the APtN<\/jats:styled-content> were evaluated in rats before and after chronic constriction injury of the sciatic nerve.<\/jats:p><\/jats:sec>Results<\/jats:title>The tactile hypersensitivity was characterized by an initial phase (the 2 days following the injury) and a maintenance phase (the subsequent 7 days). The injection of 2% lidocaine (0.25\u2009\u03bcL<\/jats:styled-content>) or N<\/jats:styled-content>\u2010methyl\u2010D<\/jats:styled-content>\u2010aspartate (2.5\u2009\u03bcg\/0.25\u2009\u03bcL<\/jats:styled-content>) into the APtN<\/jats:styled-content> intensified the tactile hypersensitivity observed 2 days after injury but did not alter the tactile hypersensitivity observed 7 and 14 days after injury. The injection of naloxone (10\u2009ng\/0.25\u2009\u03bcL<\/jats:styled-content>) or methysergide (40\u2009pg\/0.25\u2009\u03bcL<\/jats:styled-content>) but not atropine (100\u2009ng\/0.25\u2009\u03bcL<\/jats:styled-content>) into the APtN<\/jats:styled-content> also intensified the tactile hypersensitivity observed 2 days after the injury. A significant increase in the number of F<\/jats:styled-content>os\u2010ir cells was found in the contralateral APtN<\/jats:styled-content> 2 days but not 7 or 14 days after the injury. Electrical stimulation of the APtN<\/jats:styled-content> reduced the tactile hypersensitivity at 2, 7 and 14 days after the nerve ligation.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>APtN<\/jats:styled-content> exerts a tonic inhibitory influence on persistent pain. The results point out to an important role of opioid and serotonergic mediation into the APtN<\/jats:styled-content> to inhibit hyperalgesia during the initial phase of neuropathic pain.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ejp.638","type":"journal-article","created":{"date-parts":[[2014,12,9]],"date-time":"2014-12-09T07:20:52Z","timestamp":1418109652000},"page":"1148-1157","source":"Crossref","is-referenced-by-count":10,"title":["Descending mechanisms activated by the anterior pretectal nucleus initiate but do not maintain neuropathic pain in rats"],"prefix":"10.1002","volume":"19","author":[{"given":"A.C.","family":"Rossaneis","sequence":"first","affiliation":[{"name":"Department of Pharmacology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Genaro","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q.M.","family":"Dias","sequence":"additional","affiliation":[{"name":"Oswaldo Cruz Foundation Fiocruz Rond\u00f4nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.M.","family":"Guethe","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.S.","family":"Fais","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.A.","family":"Del Bel","sequence":"additional","affiliation":[{"name":"Department of Morphology, Estomatology and Physiology Faculty of Odontology of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.A.","family":"Prado","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-4328(01)00254-6"},{"key":"e_1_2_9_3_1","first-page":"18P","article-title":"An extra\u2010fine assembly for intracerebral microinjection","volume":"305","author":"Azami J.","year":"1980","journal-title":"J Physiol"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(88)90209-6"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2005.05.068"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-12-05129.2002"},{"key":"e_1_2_9_7_1","first-page":"299","article-title":"Fos, nociception and the dorsal horn","volume":"77","author":"Coggeshall R.E.","year":"2005","journal-title":"Prog Neurobiol"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431X20122462"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.2007.00474.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(99)00219-5"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.10054"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2011.06.017"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(92)90041-9"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(00)00251-7"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(98)00048-3"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(99)00185-2"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(00)01338-0"},{"key":"e_1_2_9_18_1","first-page":"107","volume-title":"Neuropathic Pain: Pathophysiology and Treatment","author":"Ossipov M.H.","year":"2001"},{"key":"e_1_2_9_19_1","volume-title":"The Rat Brain in Stereotaxic Coordinates","author":"Paxinos G.","year":"2005"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(02)02157-4"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1999.00494.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(91)90358-U"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1987.sp016499"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(93)90072-W"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp021038"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(02)00368-8"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2011.08.024"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)01025-6"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(02)00449-9"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2007.01.021"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(98)00212-7"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(83)90201-4"}],"container-title":["European Journal of Pain"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fejp.638","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ejp.638","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T12:59:44Z","timestamp":1696337984000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ejp.638"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,8]]},"references-count":31,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1002\/ejp.638"],"URL":"https:\/\/doi.org\/10.1002\/ejp.638","archive":["Portico"],"relation":{},"ISSN":["1090-3801","1532-2149"],"issn-type":[{"value":"1090-3801","type":"print"},{"value":"1532-2149","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,8]]}}}