{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:30:38Z","timestamp":1787063438111,"version":"build-2736575974"},"reference-count":80,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T00:00:00Z","timestamp":1775865600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Pain"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Emerging evidence suggests a role for non\u2010neuronal cells in the pathophysiology of chronic pain. Chronic pain causes profound alterations of the transcriptomic program of oligodendroglia, but the effect of pain on the oligodendroglial cells themselves remains unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Male and female C57BL6\/J mice underwent spared nerve injury (SNI). Mechanical hypersensitivity was assessed 5\u2009weeks later using the von Frey test. Six weeks post\u2010surgery, mice were perfused, brains dissected, and immunostained for oligodendrocyte precursor cells (OPC) and mature oligodendrocytes (OL) in four brain regions involved in pain chronification: anterior cingulate cortex (ACC), central amygdala (CeA), basolateral amygdala (BLA), and periaqueductal grey (PAG).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We found OL were reduced in the ACC and CeA of both sexes 6\u2009weeks after SNI. Conversely, BLA OL were increased in both sexes following SNI. There was a sex\u2010dependent effect of SNI on PAG\u2010OL, where OL were only reduced in females. SNI did not affect OPC in any of the studied brain regions, but female PAG and BLA appeared to have fewer OPC than males independent of SNI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Long\u2010term nerve injury differentially affects OL in a brain region\u2010 and sex\u2010dependent manner. This effect is observable 6\u2009weeks after injury, suggesting a long\u2010lasting impact of chronic pain on oligodendroglial cells. OPC, on the other hand, are remarkably stable. This finding aligns with previous literature showing OPC maintain homeostasis, even in pathological conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Significance<\/jats:title>\n                    <jats:p>Oligodendrocytes ensheathe axons to increase conduction speed, stabilize neuronal connections, and fine\u2010tune neuron\u2010to\u2010neuron communication. Furthermore, pharmacological stimulation of myelination improves pain\u2010induced cognitive deficits in mice, suggesting therapeutic potential in targeting oligodendrocytes in pain. A better understanding of how pain impacts oligodendroglia is thus crucial to better understand the pathophysiology of pain and identify new therapeutic targets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ejp.70265","type":"journal-article","created":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T12:06:42Z","timestamp":1775909202000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Spared Nerve Injury Induces Long\u2010Term and Brain Region\u2010Specific Changes in Oligodendrocyte Density in Mice"],"prefix":"10.1002","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5995-6088","authenticated-orcid":false,"given":"L\u00e9a J.","family":"Becker","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Center for Clinical Pharmacology, Washington University Pain Center Washington University in St. Louis  St. Louis Missouri 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