{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:59:14Z","timestamp":1790150354586,"version":"4.0.1"},"reference-count":69,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Pain"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Neuropathic pain is associated with anxiety\u2010like disorders, suggesting shared neurobiological mechanisms. Glutamatergic dysregulation and impaired astrocytic glutamate uptake, particularly via the astrocytic transporter GLT\u20101, have been implicated in central sensitization and associated affective disorders. Ceftriaxone, a \u03b2\u2010lactam antibiotic known to enhance GLT\u20101 expression, may represent a potential strategy to alleviate pain\u2010related anxiety.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Neuropathic pain was induced in mice using partial sciatic nerve ligation (PSNL). Ceftriaxone (200\u2009mg\/kg\/day) or saline was administered intraperitoneally. Mechanical allodynia and thermal hypersensitivity were assessed using the von Frey and Hargreaves tests. Anxiety\u2010like behaviour was evaluated in the elevated plus maze and an open field. Central sensitization was examined through c\u2010Fos expression in dorsal spinal cord sections. Microglial and astrocytic activation were analysed by immunohistochemistry, while GLT\u20101 expression was assessed by RT\u2010qPCR and Western blot.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Preventive ceftriaxone treatment reduces mechanical allodynia, thermal hypersensitivity and the development of anxiety\u2010like behaviour following PSNL. In the elevated plus maze, ceftriaxone treatment prevented the increase in closed\u2010arm exploration and resting time while preserving head\u2010dipping behaviour. In the open field, ceftriaxone\u2010receiving mice preserved centre exploration behaviour compared to saline\u2010treated mice. These behavioural effects were accompanied by increased GLT\u20101 expression in the spinal cord that coincided with a reduced lesion\u2010induced c\u2010Fos expression and a decreased microglial and astrocytic activation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Ceftriaxone mitigates neuropathic pain and its associated anxiety\u2010like phenotype through modulation of glutamatergic signalling, attenuation of central sensitization and reduction of glial reactivity. These findings support GLT\u20101 regulation as a promising therapeutic target in pain\u2010related affective disorders.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Significance Statement<\/jats:title>\n                    <jats:p>Neuropathic pain is often associated with anxiety, but the underlying mechanisms remain unclear. Increasing evidence suggests that disrupted glutamatergic homeostasis contributes to both conditions. Here, we evaluated ceftriaxone, a \u03b2\u2010lactam antibiotic that prevents the downregulation of the astrocytic glutamate transporter GLT\u20101, in a mouse model of neuropathic pain induced by partial sciatic nerve ligation. Ceftriaxone reduced both pain hypersensitivity and anxiety\u2010like behaviour, supporting glutamate homeostasis as a promising therapeutic target.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ejp.70357","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:32:21Z","timestamp":1787837541000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preventive Treatment With Ceftriaxone Attenuates Neuropathic Pain and Anxiety\u2010Like Behaviour in a Mouse Model of Partial Sciatic Nerve Ligation"],"prefix":"10.1002","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2433-4749","authenticated-orcid":false,"given":"Pauline","family":"Braconnier","sequence":"first","affiliation":[{"name":"Institute of Neuroscience, Group of Neuropharmacology Universit\u00e9 catholique de Louvain (UCLouvain)  Brussels 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